We narrowed to 157,076 results for: Des

1. pR26 iBFP Dest

   Plasmid

   #74284

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-Cys-SpyCatcher002

   Plasmid

   #102829

   Purpose

   Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Cys-SpyCatcher002
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Cysteine residue in N-terminal region

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2/v5-DEST hGlut2

   Plasmid

   #18086

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut2 (SLC2A2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

   Plasmid

   #124903

   Purpose

   Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

   Depositor

   Markus Covert

   Article

   Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

   Insert

   p65 promoter- p65 CDS-mRuby2
   

   Use

   Lentiviral

   Tags

   mRuby

   Expression

   Mammalian

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST14-DoubleCatcher-H-Lock

   Plasmid

   #216285

   Purpose

   Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher H-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTripz NEO DEST

   Plasmid

   #209072

   Purpose

   Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

   Depositor

   Matthew Oser

   Article

   Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector to be used…

   Tags

   3X FLAG

   Promoter

   CMV

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-EGFP_Hu_p115-RhoGEF(WT)

   Plasmid

   #136337

   Purpose

   Mammalian expression of human p115-RhoGEF with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   ARHGEF1 (ARHGEF1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Rab13 in pCSDEST2

   Plasmid

   #140879

   Purpose

   Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab13 (rab13 Zebrafish)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK082 desmaMCS:minprommCherry,exorh:mCerulean

    Plasmid

    #200015

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6/V5-DEST-ASF1B

    Plasmid

    #31205

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    ASF1B (ASF1B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3.2/V5-DEST-Noa1

    Plasmid

    #191969

    Purpose

    Overexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5.

    Depositor

    Luca Scorrano

    Insert

    Noa1
    

    Use

    Synthetic Biology

    Tags

    V5

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJiaDEST-APEX2-GAL9

    Plasmid

    #149520

    Purpose

    Express APEX2-Galectin-9 in mammalian cells

    Depositor

    Vojo Deretic

    Article

    Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

    Insert

    LGALS9 (LGALS9 Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6/V5-DEST-HMGB1

    Plasmid

    #31208

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCK036 desmaMCS:minCerulean,cryaa:Venus

    Plasmid

    #195981

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6/V5-DEST-MCM7

    Plasmid

    #31212

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    MCM7 (MCM7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

17. LbCas12a-2C-NLS in pCSDest

    Plasmid

    #126638

    Purpose

    Expresses LbCas12a-2C-NLS in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid

    #100025

    Purpose

    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Tags

    V5 Epitope Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-DEST-His

    Plasmid

    #119763

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Tags

    10xHis and GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST47-ARL3-GFP

    Plasmid

    #67397

    Purpose

    Mammalian expression of hArl3 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only