We narrowed to 1,358 results for: pEGFP-c1

1. pCMV-DeAct-SpvB

   Plasmid

   #89446

   Purpose

   Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-SBP-VAP-A*

   Plasmid

   #120175

   Purpose

   Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   VAMP associated protein A (VAPA Human)
   

   Tags

   EGFP and SBP

   Expression

   Mammalian

   Mutation

   Truncated VAP-A: 118-249

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. cpVenus-Gγ2

   Plasmid

   #69626

   Purpose

   The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   cp173Venus-Ggamma2 (GNG2 Synthetic)
   

   Tags

   ciruclar permutated Venus (cp173V)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-IpgD

   Plasmid

   #183666

   Purpose

   N-terminally tagged Shigella flexneri IpgD for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-cdc42palm

   Plasmid

   #20141

   Depositor

   Alaa El-Husseini

   Article

   Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

   Insert

   cell division cycle 42 isoform 2 (CDC42 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

6. GFP-cdc42prenyl

   Plasmid

   #20142

   Depositor

   Alaa El-Husseini

   Article

   Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

   Insert

   cell division cycle 42 isoform 1 (CDC42 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

7. Myc-E-Syt3

   Plasmid

   #66835

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt3 (ESYT3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. FRB-GFP-Gab1(Y628F/Y660F)

   Plasmid

   #188658

   Purpose

   FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 1 (Gab1 Mouse)
   

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y628F and Y660F

   Promoter

   CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-DroshaS300E/S302D

   Plasmid

   #62531

   Purpose

   Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 300 to glutamic acid and serine 3…

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. mEosEM-E_Lamin A

    Plasmid

    #226338

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    Lamin A (LMNA Human)
    

    Tags

    mEosEM-E

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB1N-LZ-mCherry-LOV2(wt)

    Plasmid

    #107693

    Purpose

    N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB1N-LZ-LOV2(wt)

    Plasmid

    #107614

    Purpose

    N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Tags

    A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIBN-tagBFP-hTRF2

    Plasmid

    #103803

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRB-GFP-Gab2(Y604F/Y634F)

    Plasmid

    #188659

    Purpose

    FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    GRB associated binding protein 2 (Gab2 Mouse)
    

    Tags

    FRB and GFP

    Expression

    Mammalian

    Mutation

    Y604F and Y634F

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEB1N-LZ-LOV2(fast)

    Plasmid

    #107690

    Purpose

    N-terminal half of π-EB1 with fast LOV2, no fluorescent tag

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Tags

    A. sativa phototropin 1 LOV2 domain (I427V, H519L…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    CMV

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lck-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129627

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    Lck-cpmTq2-Calcium-lifetime-sensor
    

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid

    #101300

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-509, 670-698, and 699-906 …

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NES-EGFP-cPHx2

    Plasmid

    #116854

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem dimer)

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SspB-HaloTag-DHPH-ITSN1

    Plasmid

    #176113

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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