We narrowed to 1,343 results for: pEGFP-c1
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Plasmid#62531PurposeSerine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acidDepositorInserthuman Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to glutamic acid and serine 3…PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB
Plasmid#183657PurposeN-terminally tagged Salmonella Typhimurium SopB for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-mCherry-LOV2(wt)
Plasmid#107693PurposeN-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule endsDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRB-GFP-Gab2(Y604F/Y634F)
Plasmid#188659PurposeFRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to PheDepositorInsertGRB associated binding protein 2 (Gab2 Mouse)
UseTagsFRB and GFPExpressionMammalianMutationY604F and Y634FPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(wt)
Plasmid#107614PurposeN-terminal half of π-EB1 with wild-type LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain and GCN4 leuc…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-alpha-E-Catenin 3xFP4 (895)
Plasmid#67936PurposeMammalian expression of alpha E cateninDepositorInsertalpha E catenin (Ctnna1 Mouse)
UseTags3xFP4 and EGFPExpressionMammalianMutationN-terminal domainPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(fast)
Plasmid#107690PurposeN-terminal half of π-EB1 with fast LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain (I427V, H519L…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lck-cpmTq2-Calcium-lifetime-sensor
Plasmid#129627PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertLck-cpmTq2-Calcium-lifetime-sensor
UseTagsLckExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
aCat DeltaTS (delta570 to 670 aa in aCat TS WT)
Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx2
Plasmid#116854PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem dimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SspB-HaloTag-DHPH-ITSN1
Plasmid#176113PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab7
Plasmid#214273PurposeMammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)DepositorInsertmTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-FKBP-TPD54
Plasmid#172454PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tagDepositorInsertTPD54 (TPD52L2 Human)
UseTagsGFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
SspB-iRFP-DHPH-ITSN1
Plasmid#176117PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xnls-cpmTq2-Calcium-lifetime-sensor
Plasmid#129626PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-cpmTq2-Calcium-lifetime-sensor
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hTRF2
Plasmid#103804PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPCM2w/b delta6
Plasmid#47588DepositorInsertPMCA2w/b delta6 (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF2-tagRFP-T
Plasmid#103812PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only