We narrowed to 5,516 results for: pcas

1. pMUT2-pCaf-mCherry

   Plasmid

   #192860

   Purpose

   pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   Nanobody-CadC fusion protein and cognate promoter inducible by caffeine
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG eGFP-Oct4

   Plasmid

   #203789

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG1.1-Myc/TSSK1

   Plasmid

   #199624

   Purpose

   Expression vector of mouse TSSK1. Myc-tag at N-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tssk1 mRNA (Tssk1 Mouse)
   

   Use

   Tags

   Myc-tag

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AviMta2-3xFiBsd

   Plasmid

   #140964

   Purpose

   full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

   Depositor

   Brian Hendrich

   Article

   Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   metastasis-associated gene family, member 2 (Mta2 Mouse)
   

   Use

   Tags

   3xFLAG, Avi, and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9-EF-Gsk3b(new)

   Plasmid

   #122341

   Purpose

   Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Gsk3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-GCaMP2-actin

   Plasmid

   #18928

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   GCaMP2-Actin (ACTB Chicken)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

7. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. DN1-Gli3 in AINGpCAGGS

   Plasmid

   #121970

   Purpose

   medium Gli3 transcriptional activator

   Depositor

   James Briscoe

   Article

   Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

   Insert

   DN2Gli3 (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus deletion (nt1-864)

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCaSpeR-act5cB-GAL4QF

    Plasmid

    #61309

    Purpose

    Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    GAL4_DBD::QF2w_AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Promoter

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-Omicron.BA.5-RBD

    Plasmid

    #213478

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/Omicron.BA.5 RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9-mCherry-HIST1H4C

    Plasmid

    #66944

    Purpose

    CRISPaint target selector HIST1H4C

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA HIST1H4C
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-3xHAtag-SP-PTPRO

    Plasmid

    #191696

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of PTPRO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-eArch3.0-mRuby2-ST

    Plasmid

    #109135

    Purpose

    Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eArch3.0-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS/EG5-RBD

    Plasmid

    #213410

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/EG5-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid

    #172825

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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