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Showing: 1221 - 1240 of 11564 results

  1. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  6. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

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  9. pGA1-EF1α-sfGFP150TAG-4xtRNA

    Plasmid
    #251517
    Purpose
    Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021
    Insert
    sfGFP150TAG
    Tags
    His6 and V5
    Expression
    Mammalian
    Mutation
    150 TAG (amino acid 150 mutated to stop codon)
    Promoter
    EF1alpha
    Available Since
    March 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    251517-plasmid-map-sequence-id-502878

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Showing: 1221 - 1240 of 11564 results