We narrowed to 6,273 results for: nls

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pRM053 His-TEV-Cas9 triNLS 0C

Plasmid

#196244

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

Plasmid

#133802

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA5

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls miR-122 3x

Plasmid

#35644

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

AAV

Promoter

CB

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-2xflox-BRX-eGFP-NLS

Plasmid

#217535

Purpose

AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

4X BRE reporter and miniXon casette (Smad1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

BMP response element (BRE)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

Plasmid

#134341

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC3

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3xSNAP-NLS

Plasmid

#236388

Purpose

Overexpression of PCP-3xSNAP in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

PCP-3xSNAP

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTAL-MajSat-FLAG-NLS-VP64

Plasmid

#69074

Purpose

Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTAL-MajSat-FLAG-NLS-MCS

Plasmid

#69073

Purpose

Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-NLS-Cre-2A-mCherry-FKF

Plasmid

#119868

Purpose

Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Tags

mCherry

Expression

Bacterial

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE NLS

Plasmid

#82466

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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hSyn Marina-T2A-nls-mCherry

Plasmid

#85843

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

GEVI Marina

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

hSyn1

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

Plasmid

#134337

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Efa

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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iC10 PB-zim3-mycNLS-mApple-hygro

Plasmid

#204719

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bi-ctd-Cas9n-UGI-NLS

Plasmid

#109426

Purpose

Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Use

CRISPR

Expression

Mammalian

Mutation

Insertion of an L1 intron into the coding sequenc…

Promoter

CMV

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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LLP453 pEf1a-NLS-aGCN4-mCherry

Plasmid

#100945

Purpose

mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, mCherry

Tags

3xTy1

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEN716 - pCAGGS-eGFP-3Xnls-bpA

Plasmid

#156435

Purpose

For transient expression of nuclear eGFP in mammalian cells

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

eGFP

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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