We narrowed to 5,868 results for: nls

1. pMT-MCS-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80059

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3

   Plasmid

   #122092

   Purpose

   AAV plasmid for expressing rsCaMPARI in neurons, nucleus localized

   Depositor

   Eric Schreiter

   Article

   Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

   Insert

   rsCaMPARI
   

   Use

   AAV

   Tags

   NLS-His and mRuby3

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 868 pSG5L HA NLS PTEN G129R

   Plasmid

   #10935

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   G129R

   Promoter

   Available since

   Jan. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

4. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

   Plasmid

   #127365

   Purpose

   Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   R118G

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

   Plasmid

   #115139

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3.1 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-HOXD13-IDR-YFP-NLS

   Plasmid

   #145277

   Purpose

   Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13-IDR (HOXD13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLIK 3XFLAG-MKK4-EE-NLS hygro

   Plasmid

   #87779

   Purpose

   Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

    Plasmid

    #101105

    Purpose

    Encodes dCas9(C) (without VP64) fused to VEGFR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR1-dCas9(C)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ubc-nls-pcp-5xSunTag (SRv1)

    Plasmid

    #185797

    Purpose

    pcp-5xSunTag component for SunRISER SRv1

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    pcp-5xSunTag
    

    Use

    Tags

    FactorXa site, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106352

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-AICD-NLS-IRES-hrGFP

    Plasmid

    #107543

    Purpose

    AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD-NLS (APP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

    Plasmid

    #81206

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

    Plasmid

    #113857

    Purpose

    For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    Humanized Beta
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    "humanized" Beta gene

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid

    #115140

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid

    #115138

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

    Plasmid

    #81247

    Purpose

    Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

    Plasmid

    #49313

    Purpose

    Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Insert

    nls-mCherry-Flag
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge