2,172 results

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pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

Plasmid

#229691

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
mutant cavin1b (Del4UC1) (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Mutation

Del4UC1

Promoter

LTR viral promoter

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP

Plasmid

#229690

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
cavin1b (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Promoter

LTR viral promoter

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-GATA1

Plasmid

#194436

Purpose

In Situ hybridization probes

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

GATA1a (gata1a Zebrafish)

Use

In situ hybridization

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 β-Globin

Plasmid

#194437

Purpose

In Situ hybridization probes

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

β-globin

Use

In situ hybridization

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-G-G mm

Plasmid

#221728

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-G-G mm

Use

Fish expression

Mutation

G-G mismatch of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-10-11 mm

Plasmid

#221727

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-10-11 mm

Use

Fish expression

Mutation

Mismatch at positions 10 and 11 of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430

Plasmid

#221726

Purpose

Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#227773

Purpose

Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and tp1

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#226834

Purpose

Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

Olactb and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTol1 - 14xUAS:CaSR-EGFP

Plasmid

#191502

Purpose

A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol1 transgenes…

Promoter

14X UAS

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-hcar1-4-mKate2

Plasmid

#226712

Purpose

overexpression red fluorescent hcar1-4 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

hcar1-4

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P--Dr-aCateninFL

Plasmid

#191740

Purpose

Expresses FL D rerio catenin in bacteria

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (ctnna1 Zebrafish)

Tags

GST

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-5.0dand5:EGFP

Plasmid

#223327

Purpose

Expresses EGFP in Kupffer's vesicle cells in zebrafish embryos

Depositor

Hiroyuki Takeda

Article

Ikeda et al Dev Growth Differ. 2022 Apr;64(3):163-17

Insert

5.0kb dand5 promoter (dand5 Zebrafish)

Use

Zebrafish expression

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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