2,189 results

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pTol2-cmlc2-nGFP-nCas9

Plasmid

#238308

Purpose

Expression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

Cas9 (Csn1) endonuclease from Streptococcus pyogenes

Use

Tol2-based plasmid

Tags

nGFP-P2A

Promoter

cmlc2 (myl7)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mTFP1-Rab5c

Plasmid

#244031

Purpose

fusion of Rab5c to mTFP1

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

mTFP1-Rab5c

Expression

Mammalian

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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p5E itga11a-cFos

Plasmid

#240524

Purpose

5' Gateway Entry vector containing the itga11a-cFos promoter for fibroblast-specific expression in zebrafish

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Inserts

integrin α11a - 3.2kb promoter (itga11a Zebrafish)
cFos - 216bp minimal promoter (Fos Mouse)

Use

Unspecified

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-QUAS:cPla2-mKate2

Plasmid

#241913

Purpose

Zebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUAS enhancer driving cPla2-mKate2

Use

Zebrafish tol2 transgenesis

Tags

cPla2 fused to mKate2

Promoter

QUAS

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+her3WT-P2A-sfGFP

Plasmid

#240545

Purpose

Wild-type zebrafish Her3 (ortholog to human HES3) in a -2A-sfGFP backbone for visualization

Depositor

Genevieve Kendall

Article

Kent et al Dev Biol. 2023 Apr;496:1-14. doi: 10.101

Insert

her3 (her3 Zebrafish)

Expression

Mammalian

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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apoBb.1-Dendra2

Plasmid

#218180

Purpose

used for TALEN HDR to generate endogenous fusion of HA-Dendra2 to apoBb.1 in zebrafish

Depositor

Steven Farber

Article

Moll et al Arterioscler Thromb Vasc Biol. 2025 Aug

Insert

apoBb.1-Dendra2

Use

TALEN

Tags

fuses HA-Dendra2 to apoBb.1

Mutation

inserts HA-Dendra2 sequence at C-terminus of apoB…

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf nhsl1b-mNeongreen

Plasmid

#233883

Purpose

mNeongreen tagged form of zebrafish nhsl1b. For in vitro transcription (SP6) or expression through CMV.

Depositor

Nicolas David

Article

Escot et al Commun Biol. 2025 Feb 28;8(1):328. doi:

Insert

nhsl1b (nhsl1b Zebrafish)

Use

In vitro synthesis of mrna

Tags

mNeongreen

Expression

Mammalian

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS

Plasmid

#238298

Purpose

For RNA injection of Nanog-mNG-KS into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS-FtoG

Plasmid

#238297

Purpose

For RNA injection of Nanog-mNG-KS-FtoG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS-FtoG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG

Plasmid

#238296

Purpose

For RNA injection of Nanog-mNG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

Plasmid

#238372

Purpose

Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

cmlc2 (myl7)

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-Spe-KpnI-ubb-nmKate

Plasmid

#238373

Purpose

Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

ubiquitin

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1b-FLAG

Plasmid

#232144

Purpose

Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_4ACJ

Plasmid

#234212

Purpose

Heterologous protein expression of Wufb25h12 in Escherichia coli

Depositor

Christopher Bahl

Insert

4ACJ (oxr1a Zebrafish)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_1KQX

Plasmid

#234181

Purpose

Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli

Depositor

Christopher Bahl

Insert

1KQX (rbp2a Zebrafish)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-CMV-zdgat2FLAG-SV40

Plasmid

#232141

Purpose

Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Zebrafish expression

Tags

3xFLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1a-FLAG

Plasmid

#232143

Purpose

Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ mogat3b-FLAG

Plasmid

#232145

Purpose

Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Crestin-pro (JDW 1320)

Plasmid

#229830

Purpose

A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Crestin promoter, c-fos minimal promoter, and b-globin intron

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zdgat2-CDS-FLAG

Plasmid

#232142

Purpose

Gateway middle entry vector containing zebrafish dgat2 with a C-terminal 3xFLAG tag

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Gateway cloning middle entry

Tags

3xFLAG

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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