We narrowed to 9,223 results for: Coli
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Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-T46C
Plasmid#110543PurposeBacterial expression of E.coli DHFR C85A/C152S/T46C mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationT46 mutated to C46, C85 mutated to A85, C152 muta…PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-L54C
Plasmid#110542PurposeBacterial expression of E.coli DHFR C85A/C152S/L54C mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationL54 mutated to C54, C85 mutated to A85, C152 muta…PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PatoK-OG241
Plasmid#72840PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoL-LOG241
Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
UseTagsExpressionBacterialMutationPromoterPatoLAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoK-LOG241
Plasmid#72848PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/purC
Plasmid#73623PurposeProduces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tagDepositorInsertphosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationContains multiple silent mutations (E179, G185, G…PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD419
Plasmid#63754Purpose3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.DepositorInsertehxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx
Plasmid#58714PurposeExpresses T7-Notexin in Escherichia coliDepositorInsertNotexin
UseTagsT7 peptide tagExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlum
Plasmid#119829PurposeProduction of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlum
UseTagsGSTExpressionBacterialMutationR24KPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pST44
Plasmid#64007Purposepolycistronic cloning vector for pST44 plasmid suiteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pF
Plasmid#42519DepositorTypeEmpty backboneUseUnspecified; CloningTagsExpressionBacterialMutationPromoterT7, T3Available sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-hphMX6
Plasmid#19342DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTP223
Plasmid#13263DepositorInsertPlac-gam-bet-exo
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOPINS3C
Plasmid#41115DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINNUSA
Plasmid#41119DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
P48
Plasmid#212155PurposePlasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.DepositorInsertsuperfolder green fluorescent protein, secondary alcohol dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINHALO7
Plasmid#41117DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
PIA586
Plasmid#104399Purposevector for expression of single E.coli RNAP subunitDepositorInsertrpoD
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP12049
Plasmid#222355PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPabstBRAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINMSYB
Plasmid#41118DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINTF
Plasmid#41114DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNO4
Plasmid#101066PurposeBphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINTRX
Plasmid#41116DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)
Plasmid#86933PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
RP1B
Plasmid#26563DepositorTypeEmpty backboneUseTagsHis6, TEV cleavage site, and Thio-6ExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF2
Plasmid#42520DepositorTypeEmpty backboneUseUnspecified; CloningTagsExpressionBacterialMutationPromoterT7, T3Available sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only