We narrowed to 18,106 results for: URE

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pET-28a-hPKM1

Plasmid

#44241

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M1 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a-hPKM2

Plasmid

#44242

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

Plasmid

#60660

Purpose

Flp-dependent expression of mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX-mNaChBacMut-T2A-tdTomato

Use

AAV

Mutation

E191K

Promoter

EF1α

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pWUR702

Plasmid

#53079

Purpose

Inducible TtAgo expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Tags

Strep-II tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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bactin2:loxP-STOP-loxP-DsRed-express

Plasmid

#68797

Purpose

For making transgenic fish

Depositor

David Traver

Article

Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

Inserts

beta-actin promoter
loxp-stop-codon-loxp-dsred

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

Plasmid

#60658

Purpose

Flp-dependent expression of mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Has Service

AAV8

Insert

FLEX-mNaChBac-T2A-tdTomato

Use

AAV

Promoter

EF1α

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGImC_Empty

Plasmid

#92015

Purpose

Empty backbone, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p5E mpeg1

Plasmid

#135199

Purpose

Macrophage specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 promoter

Use

Synthetic Biology

Promoter

mpeg1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRAF-MutS

Plasmid

#86605

Purpose

E. coli MutS expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutS (D835R,R840E)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

D835R, R840E

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTH3

Plasmid

#84453

Purpose

shuttle plasmid for sarA P1-YFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

YFP venus

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMM310-Myc

Plasmid

#80053

Purpose

Expression of a Myc tagged version of the BlaM-Vpr fusion protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-BlaM-Vpr

Tags

Myc

Expression

Mammalian

Mutation

N-terminal Myc tag added to construct pMM310

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJS167

Plasmid

#48881

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Inserts

araC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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FlucDM-HA-GFP11-N1

Plasmid

#91956

Purpose

Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucDM-HA-GFP11

Expression

Mammalian

Mutation

R188Q,R261Q

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-VSV-G-Myc

Plasmid

#80054

Purpose

Expression of a Myc tagged VSV-G protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VSV-G-Myc

Tags

Myc

Expression

Mammalian

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Tags

His6-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mscv2.2-6mycFlaA

Plasmid

#60203

Purpose

6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

FlaA

Use

Retroviral

Tags

6xMYC

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA-Venus

Plasmid

#186372

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltCasp1-37-GFP

Plasmid

#234069

Purpose

Encodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltCasp1-37-GFP

Use

Lentiviral

Expression

Mammalian

Mutation

Melt(C292A,Q355N); hcaspase1(R391K)

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP-Vpr-RR

Plasmid

#83374

Purpose

Expression of Myc tagged GFP-Vpr fusion protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP-Vpr

Tags

Myc and Myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCN34e-ktrA-yfp

Plasmid

#227640

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Promoter

SP6

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9ts

Plasmid

#20369

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Y68L This mutation renders Ubc9 temperature-sens…

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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