We narrowed to 7,632 results for: ALP

Showing: 2481 - 2520 of 7632 results

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pmCherry-C1-FAK-HA

Plasmid

#35039

Depositor

Anna Huttenlocher

Article

Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

Insert

focal adhesion kinase (Ptk2 Mouse)

Tags

2x HA and mCherry

Expression

Mammalian

Mutation

Y42H; deletion of amino acids 1051 & 1052

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2.aBTX

Plasmid

#122257

Purpose

Express membrane-tethered alpha-bungarotoxin in Danio rerio

Depositor

Loren Looger

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

alpha-bungarotoxin

Use

Synthetic Biology

Tags

FLAG

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)GLuc/CMV/WPREdU3

Plasmid

#136999

Purpose

Reporter vector encoding Gaussia luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Gaussia luciferase

Use

Lentiviral

Promoter

CMV

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT31

Plasmid

#18849

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT31 (YPT31 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-syb:spGFP1–10

Plasmid

#73698

Purpose

Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spGFP1–10 chimera

Use

Drosophila

Expression

Insect

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

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CFP_Rad_pcDNA4_HisMaxC

Plasmid

#41654

Depositor

Henry Colecraft

Article

Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

Insert

Rad, GTP binding proteins (RGK) (Rrad Mouse)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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FnR8-10

Plasmid

#182569

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCIG3 B

Plasmid

#132942

Purpose

MLV packaging plasmid for B-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

B-tropic MLV gag-pol

Use

Retroviral

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBRα

Plasmid

#53731

Purpose

Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

αNTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40A

Plasmid

#105304

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40A

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP704nano

Plasmid

#206192

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano-human alpha-tubulin

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRGD-TcR

Plasmid

#74110

Purpose

Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

tetA(C)

Use

Bacterial vector based on pmb1 replicon

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP670nano

Plasmid

#127441

Purpose

G protein alpha-s internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

G protein alpha-s-miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH∆1

Plasmid

#41791

Purpose

Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

HIV-1 Gag/Pol and RRE sequences

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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FnR9-10

Plasmid

#182567

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KG#62

Plasmid

#110874

Purpose

Expresses the C. elegans acy-1 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

Inserts

rab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE G1

Plasmid

#221978

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE C2

Plasmid

#221979

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE T1

Plasmid

#221980

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE T2

Plasmid

#221981

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-2mCh-PS

Plasmid

#112268

Purpose

IDOCKS Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

two copies of mCherry followed by a pseudosubstrate peptide for PKC

Tags

mCh

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL(CMV)Fluc/CMV/WPREdU3

Plasmid

#136881

Purpose

Reporter vector expression firefly luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Firefly luciferase

Use

Lentiviral

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL(CMV)EGFP/CMV/WPRE∆U3

Plasmid

#41790

Purpose

Lentiviral vector for CMV promoter driven expression of EGFP

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

EGFP

Use

Lentiviral

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TfRΔEctoR1-eGFP A206K

Plasmid

#113554

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-eGFP A206K

Expression

Mammalian

Mutation

eGFP A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pGFP Glra2 (CC#301)

Plasmid

#15199

Depositor

Connie Cepko

Article

Young et al Neuron. 2004 Mar 25. 41(6):867-79.

Insert

Glra2 (GLRA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-miR-29b-1~29a

Plasmid

#51376

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

pri-miR-29b-1~29a (MIR29B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB14

Plasmid

#18657

Depositor

Lynn Enquist

Article

Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

Insert

Us9-EGFP gene fusion

Tags

EGFP

Expression

Mammalian

Mutation

First 106 amino acids of Us9 are intact. The stop…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p1.2-Hygro

Plasmid

#162741

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2481 - 2520 of 7632 results

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We narrowed to 7,632 results for: ALP