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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab5-Blasticidin

Plasmid

#120715

Purpose

Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab5a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-215

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pBetaActin-Halo-CAMSAP3

Plasmid

#191329

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

Halo-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab11-Blasticidin

Plasmid

#120717

Purpose

Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab11a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-216

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP

Plasmid

#11772

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP (WIPF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IL1B

Plasmid

#67066

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL1B (IL1B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
MYC-NKX3.1

Plasmid

#42895

Depositor

Edward Gelmann

Article

Markowski et al Cancer Res. 2008 Sep 1;68(17):6896-901.

Insert

NKX3.1 (NKX3-1 Human)

Tags

MYC

Expression

Mammalian

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
Vh-HA-3xFKBP

Plasmid

#20222

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Vh-HA-FKBP

Plasmid

#20220

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-CPZ-LB7 vpr

Plasmid

#115834

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-LB7 Vpr (DQ373064)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts1

Plasmid

#115886

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP-VP16

Plasmid

#183917

Purpose

VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP-VP16 (UL48 Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CIBN-CAAX

Plasmid

#188989

Purpose

A lentiviral plasmid encoding plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GOR -vpr

Plasmid

#115835

Purpose

Encodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGOR Vpr (FJ424871)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p944-SWITCH-OVER insert: EF1a-Puro

Plasmid

#217891

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CALR

Plasmid

#67086

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CALR (CALR Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only