We narrowed to 10,951 results for: T7

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-Map4

Plasmid

#105571

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

MAP4 (Map4 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

Plasmid

#130640

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL703

Plasmid

#140373

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-2BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJBL728

Plasmid

#140398

Purpose

Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-csoO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL716

Plasmid

#140386

Purpose

Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-mphO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed toehold switch

Plasmid

#107353

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 1 + eforRed

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL726

Plasmid

#140396

Purpose

Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-smtO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1 tdTomato toehold switch

Plasmid

#107354

Purpose

Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 5 + tdTomato

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL724

Plasmid

#140394

Purpose

Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-hucO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1 Aqua toehold switch

Plasmid

#107356

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 8 + Aquamarine

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3

Plasmid

#171497

Purpose

T7 promotor drives in vitro transcription of mClover3 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

mClover3

Use

Pgehme

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔPRYSPRY

Plasmid

#105521

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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