We narrowed to 63,651 results for: cloning
-
Plasmid#135634PurposeAAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertChR2-mCherry
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-syn-FLEX-splitTVA-EGFP-tTA
Plasmid#100798Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19GDepositorHas serviceAAV1InsertTVA, EGFP, tet transactivator
UseAAVExpressionMammalianPromotersynapsinAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TREtight-mTagBFP2-B19G
Plasmid#100799Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorHas serviceAAV1InsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
UseAAVExpressionMammalianPromoterTREtightAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CamKIIa-jGCaMP8f-WPRE
Plasmid#176750PurposeAAV-mediated expression of GCaMP8f under the CamKIIa promoterDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
Plasmid#112677PurposeAn AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent proteinDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, and AAV RetrogradeInsertNuclear-localized floxed-mCherry EGFP
UseAAVTagsNuclear localization signalPromoterEF1aAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-SomaGCaMP6f2
Plasmid#158757PurposeCell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.DepositorHas serviceAAV8 and AAV9InsertSomaGCaMP6f2
UseAAVExpressionMammalianPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSynapsin-FLEX-soCoChR-GFP
Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas serviceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hSyn1-SIO-stGtACR1-FusionRed
Plasmid#105678PurposeExpresses a soma-targeted GtACR1 in Cre-expressing neuronsDepositorHas serviceAAV5InsertstGtACR1
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KTK_139
Plasmid#180565PurposeSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2DepositorInsertSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
ExpressionBacterialAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_324
Plasmid#180566PurposeSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2aDepositorInsertSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
ExpressionBacterialAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Voltron-ST
Plasmid#119036PurposeCre-dependant expression of soma-localized Voltron in neuronsDepositorHas serviceAAV1InsertVoltron-ST
UseAAV and Cre/LoxExpressionMammalianPromoterhsynAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CamKIIa-jGCaMP8s-WPRE
Plasmid#176752PurposeAAV-mediated expression of GCaMP8s under the CamKIIa promoterDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3
Plasmid#172921PurposeFor enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3DepositorHas serviceAAV1 and AAV9Insertaxon-jGCaMP8s-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain and P2A-mRuby3ExpressionMammalianPromoterhSynapsin1Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PiggyBac PlayBack
Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneExpressionMammalianAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a mCherry
Plasmid#129431Purposeexpression of mCherry from EF1a promoterDepositorInsertmCherry
UseLentiviralAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-neo (1795)
Plasmid#41000DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralExpressionMammalianMutationnoAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG425GAL-ccdB
Plasmid#14153DepositorPublicationTypeEmpty backboneUseGateway CloningExpressionYeastAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3basic luciferase and renilla_polyA
Plasmid#128046PurposepGL3 basic luciferase reporter with co-expression of renilla luciferaseDepositorTypeEmpty backboneExpressionMammalianAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by