We narrowed to 5,516 results for: pcas

1. pCAG-SP-CLSTN2-3xHAtag

   Plasmid

   #191693

   Purpose

   For expression coding sequencing of CLSTN2

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   signal peptide, full length coding sequence of CLSTN2 and 3xHAtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-S-B.1.1.529-FLAG

   Plasmid

   #180774

   Purpose

   B.1.1.529 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.1.529
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCas9–mKate2ps–T1gRNA

   Plasmid

   #62717

   Purpose

   t1 gRNA (ATGAGAATCAAGGCGGTCGA)

   Depositor

   Leonidas Bleris

   Article

   Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

   Insert

   Cas9–mKate2ps
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-RFP-int

   Plasmid

   #19822

   Depositor

   Zuoshang Xu

   Article

   Qiu et al BMC Biotechnol. 2008 . 8():77.

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pCAG-nDsRedFL-miRNT

   Plasmid

   #177808

   Purpose

   A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-NT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-MCS-mRuby2

   Plasmid

   #164093

   Purpose

   expression of mRuby2-tagged proteins under CAG promoter

   Depositor

   Maik Hintze

   Insert

   mRuby2
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-Nrxn1α-LNS2

   Plasmid

   #128106

   Purpose

   Express HA tagged Nrxn1α-LNS2

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS2 (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

   Plasmid

   #86611

   Purpose

   Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.2 D122A HA pCAGGS

   Plasmid

   #206097

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D122A

   Promoter

   CMV/B-actin

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid

    #172834

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-CRY2-mCherry

    Plasmid

    #178525

    Purpose

    Expresses CRY2-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-SBP-DICER1

    Plasmid

    #50558

    Purpose

    purification of Dicer1 from mammalian cells

    Depositor

    Yukihide Tomari

    Article

    Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

    Insert

    Dicer1 (Dicer1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCAG GST DEPDC5

    Plasmid

    #164983

    Purpose

    DEPDC5 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    DEPDC5 (DEPDC5 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS::AtHAP2-YFP

    Plasmid

    #132960

    Purpose

    Express AtHAP2 full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    At HAP2::YFP
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG/hArf4(WT)-mCherry

    Plasmid

    #79406

    Purpose

    Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pCaSpeR4-tubulin-QF#7m1

    Plasmid

    #46128

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7m1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-AILR-LplA-ER

    Plasmid

    #42574

    Depositor

    Alice Ting

    Article

    Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

    Insert

    LplA(AILR) with ER-retention sequence KDEL
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Compared to wild-type LplA: tryptophan 37 to alan…

    Promoter

    CAG

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-HA-Nrxn1α-LNS4

    Plasmid

    #128108

    Purpose

    Express HA tagged Nrxn1α-LNS4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS4 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-HA-Nrxn1α-LNS5

    Plasmid

    #128109

    Purpose

    Express HA tagged Nrxn1α-LNS5

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS5 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCaSpeR-act5cB-LexAQF

    Plasmid

    #61310

    Purpose

    Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA_DBD::QF2_AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge