We narrowed to 5,817 results for: pcas

1. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-nDsRedFL-miR9/9*

   Plasmid

   #177805

   Purpose

   The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-9/9*
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-B-161;+80pCalb2

   Plasmid

   #66745

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, WT)

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-puro-a5 guide B

   Plasmid

   #194011

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide B)
   

   Use

   CRISPR

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-puro-aV guide A

   Plasmid

   #194008

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide A)
   

   Use

   CRISPR

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-puro-aV guide B

   Plasmid

   #194009

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide B)
   

   Use

   CRISPR

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9n(BB)-2A-puro-a5 guide A

   Plasmid

   #194010

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide A)
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Ace2_D92N_E199V-mRuby3-ST

   Plasmid

   #129262

   Purpose

   Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Ace2_D92N_E199V-mRuby3-ST
   

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-eGtACR1-mRuby2-ST

   Plasmid

   #109136

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eGtACR1-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-Chronos-mRuby2-ST

    Plasmid

    #105446

    Purpose

    Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    Chronos-mRuby2-ST
    

    Tags

    mRuby2 and soma targeting motif

    Promoter

    pCAGGS

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAS9-mCherry-ACTG1

    Plasmid

    #66943

    Purpose

    CRISPaint target selector ACTG1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA ACTG1
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid

    #172828

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-S-B.1.617.2-FLAG

    Plasmid

    #177097

    Purpose

    B.1.617.2 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.617.2 (S SARS-CoV-2)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAG-Dcst1-3xFLAG

    Plasmid

    #183542

    Purpose

    Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

    Insert

    DC-STAMP domain containing 1 (Dcst1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-AkCas12b-2AeGFP

    Plasmid

    #121946

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-B-264;+80pCalb2

    Plasmid

    #66746

    Purpose

    luciferase reporter for Calb2 promoter (-264 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-264bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-B-419;+80pCalb2

    Plasmid

    #66747

    Purpose

    luciferase reporter for Calb2 promoter (-419 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-419bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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