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pCS2-zfIl34

Plasmid

#168112

Purpose

Express zebrafish Il34, mRNA synthesis

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Il34 (il34 Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2-WW1-mutant

Plasmid

#17795

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-WW2-mutant

Plasmid

#17796

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 to Alanine Proline 261 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST NET1A S46A

Plasmid

#69818

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

S46A

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TAG suppressor

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

Plasmid

#203965

Purpose

Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

ARID1A (Arid1a Mouse)

Use

Sleeping beauty transposon

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTrp-TGA

Plasmid

#228780

Purpose

aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTrpRS
EcWtR

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TGA suppressor

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-ORP8-H514A-H515A

Plasmid

#208360

Purpose

Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

ORP8 (OSBPL8 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H514A-H515A

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.EPC1.delta.EPcA

Plasmid

#184655

Purpose

Retroviral expression of mouse EPC1 delta EPcA

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

epc1 delta EPcA

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag.Mm.Epc1-EPcA

Plasmid

#184657

Purpose

Retroviral expression of mouse Epc1 EPcA

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Epc1-EPcA

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.DMAP1

Plasmid

#184651

Purpose

Retroviral expression of mouse Dmap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.EPC1.delta.EPcC

Plasmid

#184656

Purpose

Retroviral expression of mouse EPC1 delta EPcC

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Epc1 delta EPCc.QT

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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330-BFP-hCYP3A4-enhancer-R-sgRNA

Plasmid

#176841

Purpose

Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer right boundary

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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330-cherry-hCYP3A4-enhancer-L-sgRNA

Plasmid

#176840

Purpose

Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer left boundary

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2 gRNA resistant

Plasmid

#163408

Purpose

Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

gRNA-targeting sequences are substituted with nuc…

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R)-IRES-neo

Plasmid

#128664

Purpose

mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔEC gRNA resistant

Plasmid

#163413

Purpose

Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The extracellular domain (27-150 aa) is deleted. …

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔTail gRNA resistant

Plasmid

#163412

Purpose

Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The C-terminal tail domain (530-1038 aa) is delet…

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R)-IRES-puro

Plasmid

#128658

Purpose

mammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

Plasmid

#128666

Purpose

mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM)-IRES-hyg

Plasmid

#128668

Purpose

mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with hygromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(TM-K206A)-IRES-puro

Plasmid

#128679

Purpose

mammalian expression of Flag-Polß(TM-K206A: L301R/V303R/V306R-K206A) protein with puromycin slection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R/K206A

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(KTM)-IRES-Neo

Plasmid

#128670

Purpose

mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K41R/K61R/K81R

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

Plasmid

#128657

Purpose

mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(L301R/V306R)-IRES-puro

Plasmid

#128656

Purpose

mammalian expression of Flag-Polß(L301R/V306R) with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V306R

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(KTM)-IRES-puro

Plasmid

#128672

Purpose

mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K41R/K61R/K81R

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(K206A/K244A)-IRES-puro

Plasmid

#128683

Purpose

mammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K206A/K244A

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge