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Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEAK-Flag/INPP4B
Plasmid#24324DepositorInsertInositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
UseTagsFlag and HAExpressionMammalianMutationnonePromoterAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA2
Plasmid#174667PurposeExpresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertGFP-endoNA2 fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB122
Plasmid#68576PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His
Plasmid#83453PurposeFull-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)DepositorInsertpolycystin-1 (Pkd1 Mouse)
UseTags2xMyc, 3xFlag, and 6xHisExpressionMammalianMutationS4144DPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterGPDpAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.3
Plasmid#190797Purposeaka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final productDepositorInsert2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains2
Plasmid#78460PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2264-2315; Q2513R (see deposi…PromoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM R4114A
Plasmid#83461PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, R4113APromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB321
Plasmid#68649PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB119
Plasmid#68573PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB121
Plasmid#68575PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB222
Plasmid#68613PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB219
Plasmid#68610PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains3
Plasmid#78459PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2497-2529PromoterT7Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB221
Plasmid#68612PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB319
Plasmid#68647PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)
Plasmid#83465PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB419
Plasmid#68684PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB420
Plasmid#68685PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB422
Plasmid#68687PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB322
Plasmid#68650PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB8589
Plasmid#175221PurposeXenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYMR253C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8707
Plasmid#175222PurposeXenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYNL095C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8728
Plasmid#175223PurposeXenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOL060C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8711
Plasmid#175225PurposeXenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR092W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8713
Plasmid#175226PurposeXenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR271C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8590
Plasmid#175227PurposeXenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR307C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only