Skip to main content
Addgene
Showing: 3151 - 3200 of 18333 results

  5. pStr-KDEL_EPV-SBP-DsRed2-ER-5

    Plasmid
    #170676
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    EPV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170676-plasmid-map-sequence-id-335802

    Enlarge

  29. pUB_SM-FLuc0_EMCV-Ren

    Plasmid
    #210590
    Purpose
    Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase
    Depositor
    Olivia Rissland
    Article
    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi
    Inserts
    Spaghetti monster (FLAG)-nonoptimal FLuc
    Optimal Renilla luciferase
    Use
    Luciferase
    Tags
    Spaghetti monster (FLAG)
    Expression
    Mammalian
    Mutation
    Promoter
    EMCV IRES and UBC human
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210590-plasmid-map-sequence-id-416804

    Enlarge

  30. pUB_SM-FLuc100_EMCV-Ren

    Plasmid
    #210591
    Purpose
    Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase
    Depositor
    Olivia Rissland
    Article
    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi
    Inserts
    Spaghetti monster (FLAG)-optimal FLuc
    Optimal Renilla luciferase
    Use
    Luciferase
    Tags
    Spaghetti monster (FLAG)
    Expression
    Mammalian
    Mutation
    Promoter
    EMCV IRES and UBC human
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210591-plasmid-map-sequence-id-416807

    Enlarge

  39. pStr-KDEL_APV-SBP-Dendra2-ER-5

    Plasmid
    #170679
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    APV-SBP-Dendra2-ER-5
    streptavidin-KDEL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170679-plasmid-map-sequence-id-335798

    Enlarge

  40. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

    Enlarge

  45. pVITRO-Fab_Theo-LUCID

    Plasmid
    #119048
    Purpose
    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119048-plasmid-map-sequence-id-231811

    Enlarge

  46. pVITRO-Fab_Quin-LUCID

    Plasmid
    #119049
    Purpose
    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119049-plasmid-map-sequence-id-231810

    Enlarge

  47. pVITRO-Fab_MTX-LUCID

    Plasmid
    #119047
    Purpose
    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119047-plasmid-map-sequence-id-231808

    Enlarge

Showing: 3151 - 3200 of 18333 results