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Plasmid#45613DepositorInsertMjaTFIIB mini intein
UseTagsGB1 and H6-GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCFRSF131
Plasmid#118402Purposefor structure determinationDepositorInsertMjaTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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3x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64391Purposeencodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pVG329
Plasmid#227771PurposeFor genomic replacement of YOR1 gene in budding yeastDepositorInsertGenomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)
UseTagsExpressionYeastMutationN/APromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]
Plasmid#211891PurposeBacterial expression of CENP-T[1-242/3D]DepositorInsertCENP-T[1-242/3D] (CENPT Human)
UseTagsExpressionBacterialMutationT11D, T27D, T85DPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDKA-c009
Plasmid#175463PurposeProtein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143DepositorInsertMDK (MDK Human)
UseTagsHis6 and PTPRS (Gg) SPExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
VanR-wt
Plasmid#153339PurposeWild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoterDepositorInsertVanR
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet40
Plasmid#124358PurposeIPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1DepositorInsertgp41-1 N-intein
UseTagsHis6-GB1ExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF63
Plasmid#118403Purposefor structure determinationDepositorInsertMvuTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet33
Plasmid#118397Purposeanalysis of cis-splicingDepositorInsertMvuTFIIB intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 96-972
Plasmid#111988PurposeTruncated Hsh155 (aa 95-972)DepositorInsertHsh155 96-972
UseTagsExpressionBacterial and YeastMutation96-972 truncationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 64-972
Plasmid#111987PurposeTruncated Hsh155 (aa 64-972)DepositorInsertHsh155 64-972
UseTagsExpressionBacterial and YeastMutation64-972 truncationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 1-956
Plasmid#111999PurposeTruncated Hsh155 (aa 1-956)DepositorInsertHsh155 1-956
UseTagsExpressionBacterial and YeastMutation1-956 truncationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 1-951
Plasmid#112001PurposeTruncated Hsh155 (aa 1-951)DepositorInsertHsh155 1-951
UseTagsExpressionBacterial and YeastMutation1-951 truncationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 1-966
Plasmid#111997PurposeTruncated Hsh155 (aa 1-966)DepositorInsertHsh155 1-966
UseTagsExpressionBacterial and YeastMutation1-966 truncationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 1-961
Plasmid#111998PurposeTruncated Hsh155 (aa 1-961)DepositorInsertHsh155 1-961
UseTagsExpressionBacterial and YeastMutation1-961 truncationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 1-945
Plasmid#112002PurposeTruncated Hsh155 (aa 1-945)DepositorInsertHsh155 1-945
UseTagsExpressionBacterial and YeastMutation1-945 truncationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 1-910
Plasmid#112003PurposeTruncated Hsh155 (aa 1-910)DepositorInsertHsh155 1-910
UseTagsExpressionBacterial and YeastMutation1-910 truncationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJORSF19
Plasmid#45614DepositorInsertPhoRadA precursor
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
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p2B-56
Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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p2B-55
Plasmid#177924PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterTDH3Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GFP-HDEL
Plasmid#129098PurposeFor labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDELDepositorInsertTEF1p-SS-3xGlyAla-GFP-HDEL
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVG10
Plasmid#164702PurposeyEVenus under the control of GALL promoterDepositorInsertGALLpr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(URA)
Plasmid#49460Purposeoverespression of Flp step-arrest mutant in yeast, URA selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEDJ333
Plasmid#177272PurposeGAL1p:Cas9:CYC1tDepositorInsertCas9
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRha-rsw-EYFP
Plasmid#182928PurposeEYFP driven by inducible promoter Prha-rswDepositorInsertEYFP
UseTagsExpressionMutationPromoterPrha-rswAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSARSF53-110
Plasmid#61813PurposeTvoVMAN11 inteinDepositorInsertTvoVMAN11 intein
UseTagsH6-Smt3ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRZBA-c400
Plasmid#205109PurposeFRZB protein expressionDepositorInsertFRZB (FRZB Human)
UseTagsAviTag-6His and PTPRSspExpressionMammalianMutationWTPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-3-6AOptParkin
Plasmid#165107Purposebacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin geneDepositorInsertParkin isoform 1 (PRKN Human)
UseTagsGST-6-alanineExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAraTMDN
Plasmid#47515PurposeContains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assayDepositorInsertIntegrin alpha2B L980A TM-CYTO
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVG47
Plasmid#164705PurposeyEVenus under the control of tetOprDepositorInserttetOpr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13(315)
Plasmid#122072PurposeExpressing GFP-Atg13DepositorInsertATG13
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
HSP104-b/Leu(WT)
Plasmid#1156DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsGAL1:10 promoterExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
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NM R2E2 GFP
Plasmid#1208DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutation2 additional copies of second oligopeptide repeat…PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
RMC sup35 promotor
Plasmid#1174DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(A503V)
Plasmid#1152DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationA503VPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pVG49
Plasmid#164707PurposeyEVenus under the control of GAL1 promoterDepositorInsertGAL1pr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(Trp1)
Plasmid#49459Purposeoverespression of Flp step-arrest mutant in yeast, TRP1 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDBD-R
Plasmid#50571PurposeDouble Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572DepositorInsertDHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
UseTagsExpressionYeastMutationM1R (B-gal)PromoterGal1Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
NM R(delta) 2-5 GFP
Plasmid#1209DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationlast four repeats of sup35 N domain deletedPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pVG45
Plasmid#164703PurposeyEVenus under the control of CUP1 promoterDepositorInsertCUP1pr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only