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pET28-AviTag-DogTag-MBP

Plasmid

#171773

Purpose

Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogTag-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1

Plasmid

#19846

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
piggybac-Syn-mScn8a-3xHA

Plasmid

#182555

Purpose

Expresses Nav1.6 tagged with 3xHA

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Insert

Scn8a (Scn8a Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-VcRibB

Plasmid

#187977

Purpose

Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

Depositor

Audrey Lamb

Article

Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

Insert

VcRibB

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mGreenLantern

Plasmid

#195988

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel

Plasmid

#97008

Purpose

Rosa26 locus targeting vector

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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REEP5-EGFP

Plasmid

#210363

Purpose

Expresses REEP5 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1

Plasmid

#19838

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only

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1432 pcDNA3 flag MAGI1c

Plasmid

#10714

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

MAGI-1 (Magi1 Mouse)

Tags

flag

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagMLL2-pL-ENL(5613)

Plasmid

#20874

Depositor

Robert Slany

Article

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL2 (Kmt2d, MLLT1 Mouse, Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Article

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Expression

Mammalian

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9 T186A

Plasmid

#28101

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Contains a T186A mutation in Cdk9

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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LSM081 SFFV-mCherry-SGc(stem-1(3xMS2)2-stops)cSG-EGFP (FLP-IN)

Plasmid

#233440

Purpose

SFFV driven LIDAR reporter with an additional stop codon on the stem loop

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

SGc(stem-1(3xMS2)2-stops)cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag WDR5

Plasmid

#15553

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-RAGEdeltaCyto

Plasmid

#71436

Purpose

mammalian expression of human RAGE cytoplasmic domain mutant

Depositor

Henri Huttunen

Article

Huttunen et al J Biol Chem. 1999 Jul 9;274(28):19919-24

Insert

RAGE (AGER Human)

Expression

Mammalian

Mutation

delta 367-404

Promoter

CMV

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-FL

Plasmid

#105596

Purpose

retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-BE4

Plasmid

#108386

Purpose

Mammalian expression vector for xCas9(3.6)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-BE4

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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