We narrowed to 5,544 results for: SPI;

1. pTwist-SARS-CoV-2 Δ18 B.1.429

   Plasmid

   #169461

   Purpose

   Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.429 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Δ18 B.1.1.298

   Plasmid

   #169460

   Purpose

   Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

   Plasmid

   #104566

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-p65
   

   Use

   Tags

   3x HA and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

   Plasmid

   #104568

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x1)
   

   Use

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

   Plasmid

   #104569

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x2)
   

   Use

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. hNSE-NF186(K519TAG)-HA

   Plasmid

   #239782

   Purpose

   Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K519TAG

   Promoter

   hNSE (obtained from Addgene plasmid #11606)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hNSE-NF186(K534TAG)-HA

   Plasmid

   #239783

   Purpose

   Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K534TAG

   Promoter

   hNSE (obtained from Addgene plasmid #11606)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hNSE-NF186(K571TAG)-HA

   Plasmid

   #239784

   Purpose

   Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K571TAG

   Promoter

   hNSE (obtained from Addgene plasmid #11606)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hNSE-NF186(K604TAG)-HA

   Plasmid

   #239785

   Purpose

   Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K604TAG

   Promoter

   hNSE (obtained from Addgene plasmid #11606)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hNSE-NF186(K680TAG)-HA

    Plasmid

    #239786

    Purpose

    Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Nfasc (Nfasc Rat)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K680TAG

    Promoter

    hNSE (obtained from Addgene plasmid #11606)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hNSE-NF186(K809TAG)-HA

    Plasmid

    #239787

    Purpose

    Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Nfasc (Nfasc Rat)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K809TAG

    Promoter

    hNSE (obtained from Addgene plasmid #11606)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6xHsa.NFKB:Kaede

    Plasmid

    #239992

    Purpose

    Kaede driven by NFkB activity can be switched from green to red fluorescence by UV light exposure, enabling tracking of NFkB positive cells

    Depositor

    Raquel Espin-Palazon

    Insert

    photo convertible Kaede
    

    Use

    Tags

    NA

    Expression

    Mutation

    Promoter

    cfos minimal promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

    Plasmid

    #229827

    Purpose

    A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xnls-TagBFP2-MCS-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

    Plasmid

    #229843

    Purpose

    A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Dll4-F2 (exon3)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

    Plasmid

    #229811

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA transactivator-LexA-Operon-nls-GFP
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

    Plasmid

    #229817

    Purpose

    A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

    Plasmid

    #229823

    Purpose

    A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge