We narrowed to 10,458 results for: T7

1. pCFB8455

   Plasmid

   #175207

   Purpose

   Xenopus oocyte expression vector containing YCR075C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCR075C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YCR075C
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-hXRCC1pd-HIS

   Plasmid

   #206036

   Purpose

   Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   hXRCC1
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

   Promoter

   T7

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-RnCENP-O-GFP

   Plasmid

   #205179

   Purpose

   Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

   Insert

   CENP-O (Cenpo Rat)
   

   Use

   In vitro transcription

   Tags

   5 glycines linker followed by GFP

   Expression

   Mammalian

   Mutation

   Wild type rat CENP-O

   Promoter

   T7

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET PI-SceI C-His K301A

   Plasmid

   #195611

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A822G, A823C, A824T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEUK028

   Plasmid

   #180826

   Purpose

   T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

   Depositor

   Gregg Beckham

   Article

   Bleem et al Chem Catalysis, 2022

   Insert

   SwGdmA (SWIT_RS16490 Sphingomonas wittichii RW1, Synthetic)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEUK037

   Plasmid

   #180829

   Purpose

   T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

   Depositor

   Gregg Beckham

   Article

   Bleem et al Chem Catalysis, 2022

   Insert

   CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL714

   Plasmid

   #140384

   Purpose

   Expresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-ctcO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL710

   Plasmid

   #140380

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 14BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-14BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL702

   Plasmid

   #140372

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 0BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-0BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL706

    Plasmid

    #140376

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 6BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-6BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJBL707

    Plasmid

    #140377

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 8BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-8BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJBL708

    Plasmid

    #140378

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 10BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-10BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJBL709

    Plasmid

    #140379

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 12BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-12BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL720

    Plasmid

    #140390

    Purpose

    Expresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-qacO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBL722

    Plasmid

    #140392

    Purpose

    Expresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-ttgO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSN61

    Plasmid

    #100122

    Purpose

    expresses putative OCT4 DBD with a T7 tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT-eGFP

    Plasmid

    #215168

    Purpose

    Encodes the enhanced green fluorescent protein

    Depositor

    Travis Beddoe, Bhuvana Shanbhag

    Insert

    WT-eGFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETTEV-SENP8

    Plasmid

    #196361

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    SENP8/NEDP1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIVTR(T7A)-mTagBFP2

    Plasmid

    #172295

    Purpose

    In vitro transcription of mTagBFP2 blue fluorescent protein

    Depositor

    Patrik Verstreken

    Insert

    mTagBFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDB24

    Plasmid

    #91959

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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