We narrowed to 11,275 results for: Vars

1. pAM1160c2

   Plasmid

   #159724

   Purpose

   Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Tags

   Gal4DBD and MPGIL-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM1674

   Plasmid

   #159726

   Purpose

   Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Tags

   Gal4DBD and MPTLV-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM1479a3

   Plasmid

   #159725

   Purpose

   Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Tags

   Gal4DBD and MYITV-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX-FHH-MAPKAP1RBD

   Plasmid

   #120710

   Purpose

   Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MAPKAP1 RBD
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Amino acids 279-353

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW106

   Plasmid

   #111138

   Purpose

   For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

   Depositor

   Alexander Varshavsky

   Article

   Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

   Insert

   MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
   

   Tags

   3x Flag

   Expression

   Yeast

   Mutation

   Leu2 deletion

   Promoter

   Gal1

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETa-Amrose v2

   Plasmid

   #79538

   Purpose

   pRSETa (Invitrogen) plasmid expressing Amrose variant 2

   Depositor

   Randolph Caldwell

   Article

   Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

   Insert

   Amrose v2
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PMKO-sh_lnc-BATE1

   Plasmid

   #64866

   Purpose

   Expresses shRNA targeting lnc-BATE1

   Depositor

   Lei Sun

   Article

   Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

   Insert

   shRNA targeting lnc-BATE1
   

   Use

   Retroviral

   Promoter

   u6

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pNB786

   Plasmid

   #60151

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Promoter

   MET17pr

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNB812

    Plasmid

    #60160

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Promoter

    RNR1pr

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCell-Free_KA2_ZIKV NS2B

    Plasmid

    #145364

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2B
    

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

    Plasmid

    #239948

    Purpose

    Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    Fc2-MFSD6(L3)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CDK1as_T2A_puromycin

    Plasmid

    #118595

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-MFSD6-Puro

    Plasmid

    #239953

    Purpose

    Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-MFSD8-Puro

    Plasmid

    #239954

    Purpose

    Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD8
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBG-ttLbUV2

    Plasmid

    #231386

    Purpose

    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    ttLbCas12a Ultra V2
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D156R and E795L

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDL025_HA-rb_IgG_HC_15F11

    Plasmid

    #193622

    Purpose

    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti_HA_15F11 rabbit heavy chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pDL026_HA-rb_IgG_LC_15F11

    Plasmid

    #193623

    Purpose

    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-HA_15F11 rabbit light chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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