Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,482 results for: piggyBac

Showing: 1261 - 1280 of 1482 results

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PB-AlfaTag

Plasmid

#253248

Purpose

Empty plasmid for cloning or as control for PB-LYST-AlfaTag (Plasmid #253252) and PB-AlfaTag-MYO5A (Plasmid #253250)

Depositor

May Malicdan

Article

Serra-Vinardell et al Cell Mol Life Sci. 2023 Jan 28;80(2):53.

Type

Empty backbone

Use

Piggybac transposon

Tags

AlfaTag

Expression

Mammalian

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3156-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank1_COLO320 DM_ AT_sg-276-282])

Plasmid

#251113

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00134_PGK-mNeonGreen-syn_pA-EF1alpha-TagBFP-syn_pA

Plasmid

#252538

Purpose

Constitutive promoters with downstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00135_EF1alpha-TagBFP-syn_pA-PGK-mNeonGreen-syn_pA

Plasmid

#252539

Purpose

Constitutive promoters with upstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH

Plasmid

#252540

Purpose

Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

rtTA

Expression

Mammalian

Promoter

CMV (human cytomegalovirus immediate early enhanc…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40

Plasmid

#252541

Purpose

Inducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40)

Plasmid

#252542

Purpose

Inducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01418_(TRE-TagBFP-bGH)-EF1alpha-mRuby2-SV40

Plasmid

#252543

Purpose

Inducible gene and constitutive reporter with divergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2981-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank6_PC3 DM_AT_sg-227-233])

Plasmid

#251111

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3158-{PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank3_COLO320 DM_ AT_sg-290-296])

Plasmid

#251115

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3159-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank4_COLO320 DM_ AT_sg-297-303])

Plasmid

#251116

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3157-{PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_COLO320 DM_ AT_sg-283-289])

Plasmid

#251114

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS])

Plasmid

#251108

Purpose

Expression of dCas9 component of Casilio-i system

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

dCas9

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2977-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank1_PC3 DM_AT_sg-213-219])

Plasmid

#251109

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2978-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_PC3 DM_AT_sg-234-240])

Plasmid

#251110

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT2982-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank7_PC3 DM_AT_sg-241-247])

Plasmid

#251112

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pPig-hEF1a-Cry2PHR-LRP6c-Puro-Blind

Plasmid

#249712

Purpose

Expresses optogenetic LRP6 in mammalian cells; no fluorescent proteins. Plasmid contains a puromycin selection marker.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

Cry2-LRP6 (CRY2, LRP6 Human, Mustard Weed)
P2A-PuroR

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-shMfn1-mCherry-CAAX

Plasmid

#228740

Purpose

Membrane-tethered mCherry shRNA targeting Mfn1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Mfn1 (Mfn1, Mfn1 Mouse, Rat)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJL354

Plasmid

#243709

Purpose

A piggyBac vector containing the endogenous COX7A2 genomic locus, with its native 5' UTR replaced by the Actb 5' UTR, modified with silent mutations to allow for PCR-based analysis

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

COX7A2 genomic locus, with its 5' UTR replaced by the ACTB 5' UTR (COX7A2 Human)

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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