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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pVITRO1-102.1F10-IgM/λ

Plasmid

#61880

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB303

Plasmid

#68631

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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tdTomato-Lifeact-7

Plasmid

#54528

Purpose

Localization: Actin, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Lifeact-tdTomato

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pMito-miRFP703

Plasmid

#80000

Purpose

Mitochondria labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

cytochrome C oxidase subunit VIII (COX8A Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flpo

Plasmid

#55637

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, and AAV2

Insert

Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMaRSC

Plasmid

#89189

Purpose

The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

Flag-L274G-T2A-mCherry (Mars1 Mouse)

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS2422_Nme2.Smu-ABE8e-i1

Plasmid

#201520

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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