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pET-16b-10His-TEV-MCES_VACCA_D1_D12

Plasmid

#203671

Purpose

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

Depositor

Stephen Cusack

Article

Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

Insert

MCES_VACCA_D1_D12

Tags

10xHis

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFlagCMV2-EFP

Plasmid

#12449

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

EFP (TRIM25 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAB_14-3-3

Plasmid

#109904

Purpose

Protein expression and purification of YWHAB_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAB_14-3-3 (YWHAB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre

Plasmid

#243748

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20-Pdp1_NTD-Cre

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PGK-HA-PGRN

Plasmid

#234872

Purpose

Lentiviral plasmid expressing HA-tagged human progranulin.

Depositor

Andrew Arrant

Article

Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

Insert

Progranulin (GRN Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

PGK

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_iRFP670 sgBFP

Plasmid

#192478

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A iRFP670

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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