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pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

Plasmid

#213402

Purpose

douple floxed, myristoylation (myr) site for membrane targeting and EGFP

Depositor

Katrin Willig

Article

Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

Insert

EGFP

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

Plasmid

#115516

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Expression

Mutation

Promoter

UAS

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK-GFP

Plasmid

#83470

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

doxycycline-inducible promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-mPMEL

Plasmid

#174608

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane GFP fused to LLO and mouse PMEL antigen

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-F98 IRES EGFP

Plasmid

#130710

Purpose

Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP1 R152Q GFP

Plasmid

#51884

Purpose

expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 R152Q (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

R152Q: near the extracellular side of the fourth …

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-ST-P2A-pSC-CAAX

Plasmid

#207637

Purpose

A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX

Use

Tags

Expression

Mammalian

Mutation

Amber stop codon at SC's critical lysine

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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