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pJB03
Plasmid#111961PurposeExpresses MBP-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterTacAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_WT
Plasmid#139109PurposeExpress FL hnRNPA2 with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+H1.4
Plasmid#157796PurposeExpression of human linker histone H1.4DepositorInsertH1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
ICMAB - pET22b T7 EncFtn Mms6
Plasmid#225538PurposeConstruction of intracellular magnetite accumulating bacterial systems.DepositorInsertsIron binding protein
Ferroxidase
Material binding peptite
Iron transporter protein
UseTagsnoExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
Plasmid#101215PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1) variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A and R1060APromoterT7Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_D290V
Plasmid#139110PurposeExpress FL hnRNPA2 D290V with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationD290VPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin-GFP
Plasmid#186138PurposeFor expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and mEGFPExpressionBacterialMutationPromoterLacAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain
Plasmid#101205PurposeBacterial expression plasmid for SpCas9 REC3 domainDepositorInsertSpCas9 variant K506–Q712
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK506–Q712PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_P298L
Plasmid#139111PurposeExpress FL hnRNPA2 P298L with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationP298LPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant
Plasmid#101213PurposeBacterial expression plasmid for SpCas9 + K855A variantDepositorInsertSpCas9 variant K855A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK855APromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
polySUMO1
Plasmid#221066PurposeExpression of polySUMO1 protein under T7 promoterDepositorInsertFive repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5e-Rubicon RH N821K, T911R, K916R
Plasmid#216707PurposeBacterial protein expression of Rubicon RH domain mutantDepositorInsertRubicon (RUBCN Human)
UseTagsMBPExpressionBacterialMutationN821K, T911R, K916RPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTE_25
Plasmid#111638PurposeFunclib design PTE_25DepositorInsertPTE_25
UseTagsMBPExpressionBacterialMutationPromoterpTacAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTE_26
Plasmid#111639PurposeFunclib design PTE_26DepositorInsertPTE_26
UseTagsMBPExpressionBacterialMutationPromoterpTacAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTE_27
Plasmid#111640PurposeFunclib design PTE_27DepositorInsertPTE_27
UseTagsMBPExpressionBacterialMutationPromoterpTacAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTE_28
Plasmid#111641PurposeFunclib design PTE_28DepositorInsertPTE_28
UseTagsMBPExpressionBacterialMutationPromoterpTacAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only
