We narrowed to 5,868 results for: nls

1. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

   Plasmid

   #221537

   Purpose

   Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

   Depositor

   Omar Akbari

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1203T-CMV-Cas7.1-NLS-bGHpolyA

   Plasmid

   #221540

   Purpose

   Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

   Plasmid

   #222861

   Purpose

   This plasmid codes for the OgeuIscB nickase

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB Nickase
   

   Use

   CRISPR

   Tags

   3X HA and Nucleoplasmin NLS and ITR2

   Expression

   Mammalian

   Mutation

   E193A for nickase mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-fDIO-ArgiNLS-AausFP1

   Plasmid

   #220605

   Purpose

   Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-fDIO-ArgiNLS-mKate2

   Plasmid

   #220607

   Purpose

   Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mKate2
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-fDIO-ArgiNLS-oScarlet

   Plasmid

   #220606

   Purpose

   Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   oScarlet
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

   Plasmid

   #220597

   Purpose

   Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mVenus-Q69M (ME)
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FLEX-ArgiNLS-EGFP

   Plasmid

   #220601

   Purpose

   Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-IDR4-mCh-Cry2WT-NLS

    Plasmid

    #221920

    Purpose

    Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR4 (aa 662-787) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR4 region (aa 662-787); insert…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-CFID-mCh-Cry2WT-NLS

    Plasmid

    #221923

    Purpose

    Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP CFID (aa 1892-2441) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the CBP-FUS interaction domain (CFID…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-IDR3-mCh-Cry2WT-NLS

    Plasmid

    #221919

    Purpose

    Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR3 (aa 441-587) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR3 region (aa 441 - 587); inse…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-IDR7-mCh-Cry2WT-NLS

    Plasmid

    #221922

    Purpose

    Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR7 (aa 2109-2434) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR7 region (aa 2109 - 2434); in…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-IDR6-mCh-Cry2WT-NLS

    Plasmid

    #221921

    Purpose

    Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR6 (aa 1856-2090) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR6 region (aa 1856-2090); inse…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid

    #221918

    Purpose

    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR1 (aa 72-175) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR1 region (aa 72 - 175); inser…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid

    #219702

    Purpose

    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase

    Depositor

    Jennifer Nemhauser

    Insert

    pAHP6:PhiC31-NLS:tUBQ1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2-QUAS:NLS-mApple-he1.1:CFP

    Plasmid

    #184814

    Purpose

    Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-mApple-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-mApple

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDestTol2-QUAS:NLS-GFP-he1.1:CFP

    Plasmid

    #184815

    Purpose

    Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-GFP-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-GFP

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-2xflox-BRX-eGFP-NLS

    Plasmid

    #217535

    Purpose

    AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    4X BRE reporter and miniXon casette (Smad1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BMP response element (BRE)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIp128-TET-NUbo-E3(48)-NLS-VSV

    Plasmid

    #212746

    Purpose

    Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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