We narrowed to 9,223 results for: Coli
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Plasmid#225194PurposeUsed for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag003
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His-mPer1
Plasmid#219421PurposeExpresses 3873 bp of mPer1 coding sequence in E. coli.DepositorInsertmPer1 (Per1 Mouse)
UseTagsHis and V5ExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EcRfaHKOW-L1L2NusG
Plasmid#113126PurposeExpresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residuesDepositorInsertRfaH (rfaH Escherichia coli)
UseTagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 1-107, replaced L1 and L2 loops …PromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PatoM-OG241
Plasmid#72842PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.DepositorInsertPatoM
UseTagsExpressionBacterialMutationPromoterPatoMAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1412
Plasmid#63787PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection markerDepositorInserteCitrine-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAKTaq
Plasmid#25712PurposeBacterial expression of Taq polymeraseDepositorInsertTaq polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJMP12042
Plasmid#222354PurposeTn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFGL821
Plasmid#58223PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3aTr
Plasmid#64008PurposeSingle donor or transfer plasmid for source of translation cassette for plasmid suiteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15aC-GFP
Plasmid#107862PurposeControl plasmid expressing GFPDepositorInsertGFP
UseTagsmycExpressionBacterialMutationPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC-GST-TEV-GG
Plasmid#215795PurposeCloning of target gene with N terminal GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-NH10-GG
Plasmid#215806PurposeCloning of target gene with N terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP12089
Plasmid#222357PurposeTn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CTH10STII-GG
Plasmid#215804PurposeCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEZ-Frt-lox-DT
Plasmid#11736PurposeConditional gene targeting vector; contain cloning sites for 5′ and 3′ homology arms, and a central targeting region flanked by LoxP sites, and a neomycin cassette flanked by FRT sites.DepositorInsertpEZ-Frt-lox-DT
UseCre/Lox; /frtTagsExpressionMutationPromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHYDuet183
Plasmid#41685DepositorInsertPhoRadA intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet26
Plasmid#41689DepositorInsertTvo VMA intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIC-MBP-TEV-GG
Plasmid#215794PurposeCloning of target gene with N terminal MBP tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP12068
Plasmid#222356PurposeTn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pNIC-HIS6-SUMO1-GG
Plasmid#215799PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSG578
Plasmid#214215Purposeintegrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P81nmt1-3FLAG
Plasmid#19350DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P41nmt1-3FLAG
Plasmid#19349DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only