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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOCC154

Plasmid

#118902

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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p6640 MSCV-P C-FlagHA 16E7-Kozak

Plasmid

#35018

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV16 E7 (E7 HPV 16)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 14, 2012

Availability

Academic Institutions and Nonprofits only
YSBE 508 : pNEO_GFP

Plasmid

#92081

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pOCC153

Plasmid

#118901

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCD-Ugi22

Plasmid

#207004

Purpose

pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)

Depositor

Wayne Parrott

Article

Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

Insert

Utricularia gibba insulator 22

Use

Golden gate compatible cloning vector

Available Since

Nov. 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCD-Ugi21

Plasmid

#207005

Purpose

pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)

Depositor

Wayne Parrott

Article

Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

Insert

Utricularia gibba insulator 21

Use

Golden gate compatible cloning vector

Available Since

Nov. 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET30a-FluB-NP

Plasmid

#165093

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza B virus(B/Georgia/05/2019) nucleocapsid protein

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-EGFP-C1-HsAGO1_F

Plasmid

#146314

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Expression

Mammalian

Mutation

N574S

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLK69

Plasmid

#37258

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

lacks the 4 C-term aa

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
p6868 MSCV-IP N-V5 Zer1

Plasmid

#35100

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

Zer1 (ZER1 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pAB094e

Plasmid

#79245

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PBAD rpoZ-HA4 (Y87A); PC araC

Expression

Bacterial

Promoter

PBAD; PC

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1079

Plasmid

#108979

Purpose

Knockout cassette Blasticidin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Blasticidin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTR

Plasmid

#119859

Purpose

gRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporter

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

gRNA dual luciferase reporter

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAB045a

Plasmid

#79224

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ rpoZ-HA4; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-PI(WT)-Vav2(DPZ)

Plasmid

#86974

Purpose

PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/C1 (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDM1489

Plasmid

#108996

Purpose

Cre expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Cre recombinase

Use

Cre/Lox; Cre expression vector

Promoter

act15

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAB061i2

Plasmid

#79229

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pPI278

Plasmid

#113227

Purpose

extrachromosomal expression vector, expression of H2B-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

H2B

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Promoter

act15

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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POM42-mCherry

Plasmid

#69856

Purpose

N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Expression

Yeast

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDM1488

Plasmid

#108995

Purpose

Cre expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Cre recombinase

Use

Cre/Lox; Cre expression vector

Promoter

act15

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD6-pGRR-r11-r10-yeGFP

Plasmid

#74368

Purpose

pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

yeGFP

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

pGRR-r11-r10

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDM1082

Plasmid

#108982

Purpose

Knockout cassette G418

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette G418

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1

Plasmid

#25034

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6B (TNRC6B Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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p6656 MSCV-P C-Flag HA 25E7-Kozak

Plasmid

#35149

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pAB082b

Plasmid

#79234

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d3

Expression

Bacterial

Promoter

Ppro1

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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