We narrowed to 2,903 results for: BFP

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

   Plasmid

   #128758

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #3 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

   Plasmid

   #128756

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

   Plasmid

   #128757

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

   Plasmid

   #117137

   Purpose

   Expression vector encoding BFP-BSD-Nkx2-5

   Depositor

   Kosuke Yusa

   Article

   Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

   Insert

   Nkx2-5 CDS (Nkx2-5 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

   Plasmid

   #188777

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

   Plasmid

   #180587

   Purpose

   FdCd

   Depositor

   Won Do Heo

   Article

   Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

   Insert

   Cre
   

   Use

   AAV

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

   Plasmid

   #219548

   Purpose

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
   

   Expression

   Mammalian

   Mutation

   The (SAGG)x4 linker between GAL4(1-65) and the PC…

   Promoter

   CMVd1

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

   Plasmid

   #170273

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
   

   Expression

   Mammalian

   Mutation

   See Comments

   Promoter

   CMVd1

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158653

    Purpose

    making transgenics expressing a UAS driven mCherry and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200402

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]

    Plasmid

    #133325

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of CTD in full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221076

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221077

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221079

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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