We narrowed to 12,364 results for: NUC

1. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-Opto-Acvr1ba-P2A-Acvr2ba

   Plasmid

   #232640

   Purpose

   Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

   Depositor

   Katherine Rogers

   Insert

   Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTetOne-tdTomato-cNLS

   Plasmid

   #138529

   Purpose

   Inducible expression of tdTomato with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   tdTomato
   

   Use

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NET47 pEGFP-N2 (593)

   Plasmid

   #61986

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET47 (TM7SF2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Jill Wildonger, Melissa Harrison, Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-pDendra2(N)

   Plasmid

   #75283

   Purpose

   Expresses H2B-Dendra2 in mammalian cells

   Depositor

   Xavier Darzacq

   Article

   Recamier et al Nucleus. 2014 Jan-Feb;5(1):75-84. doi: 1

   Insert

   H2bk (H2BC12 Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSQT834

   Plasmid

   #53371

   Purpose

   Csy4 and Cas9 nuclease expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV U6+27

   Plasmid

   #25709

   Purpose

   for nuclear expression of small RNAs in mammalian cells

   Depositor

   David Engelke

   Article

   Good et al Gene Ther. 1997 Jan . 4(1):45-54.

   Insert

   U6 snRNP (LSM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7-RelA(K310R)

   Plasmid

   #23250

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   NFkB RelA (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   K310R

   Promoter

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCSC-NES-RFP

    Plasmid

    #221812

    Purpose

    Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NES-mCherry
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. human PNP non-tagged

    Plasmid

    #64199

    Purpose

    Expression of native human PNP (non-tagged)

    Depositor

    Vern Schramm

    Article

    Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCL1032

    Plasmid

    #217962

    Purpose

    Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    NASP (NASP Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT/TO/FLAG/HA-DEST TNRC6C

    Plasmid

    #19885

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6C (TNRC6C Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-p53 (NES-)

    Plasmid

    #12092

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    p53 (NES-) (TP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L348A L350A Localized to nucleus.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-H2A-TORCAR

    Plasmid

    #175016

    Purpose

    TORCAR targeted to the nucleus via histone H2A.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    H2A-TORCAR
    

    Use

    Tags

    Histone H2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SARS-CoV-2_N_deltalinker

    Plasmid

    #157870

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaliner

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SARS-CoV-2_N_deltaCTD

    Plasmid

    #157871

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaCTD

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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