We narrowed to 165,347 results for: addgene

1. p-dCas9-p300-Hygro

   Plasmid

   #104411

   Purpose

   transient expression of dCas9-p300 fusion protein

   Depositor

   Stephan Beck

   Insert

   p300 (EP300 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JMJD1CA-c089

   Plasmid

   #79644

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD1C (JMJD1C Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pINDUCER20-FLAG-hPAX3 (#735)

   Plasmid

   #78336

   Purpose

   Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

   Depositor

   Louis Kunkel, Matthew Alexander

   Insert

   PAX3 (PAX3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   TRE2

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pNM3732

   Plasmid

   #153086

   Purpose

   mec-4p GFP-C1 positive control for RMCE integration

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p GFP-C1 tbb-2 3'
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB_EF1a_HA-VSVG

   Plasmid

   #212647

   Purpose

   piggyBac vector to express HA-VSVG

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   VSVG
   

   Use

   Piggybac

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 6ASB (FBXL19)

   Plasmid

   #119954

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   FBXL19 (FBXL19 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 31-153

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

   Plasmid

   #191527

   Purpose

   Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG) + 3xFLAG_TwinStrep

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ACE2 g2

   Plasmid

   #153012

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJUNB.1.0-gDNA

   Plasmid

   #112409

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

   Depositor

   Kevin White

   Insert

   JUNB (JUNB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDB4279

    Plasmid

    #98698

    Purpose

    A control plasmid containing the intact bsdMX marker

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LEGO-AP1x3-GM55

    Plasmid

    #217414

    Purpose

    Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 6C1A (MBD2)

    Plasmid

    #119958

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD2 (MBD2 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCM130

    Plasmid

    #45828

    Depositor

    Mary Lidstrom

    Article

    Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

    Type

    Empty backbone

    Use

    Low-background broad-host-range promoter-probe ve…

    Promoter

    none

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG306-GPD-empty chr I

    Plasmid

    #41895

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ACE2 g3

    Plasmid

    #153013

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRL488

    Plasmid

    #70263

    Purpose

    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.

    Depositor

    Peter Wolk

    Article

    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.

    Type

    Empty backbone

    Use

    Luciferase; Cyanobacteria expression

    Tags

    luxAB

    Promoter

    none

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. USP21 (3MTN)

    Plasmid

    #28112

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP21 (USP21 Human)
    

    Tags

    His tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    catalytic domain only (amino acid residues 209-56…

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pLPhygCAS9

    Plasmid

    #63555

    Purpose

    Expresses CAS9 in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
    

    Use

    Leishmania expression

    Tags

    flag

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge