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We narrowed to 167,150 results for: addgene

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Showing: 1281 - 1300 of 167150 results

  2. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  3. pELK3.1.0-gDNA

    Plasmid
    #112418
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3
    Depositor
    Kevin White
    Insert
    ELK3 (ELK3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112418-plasmid-map-sequence-id-230297

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  4. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Promoter
    none
    Available Since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

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  6. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP21 (USP21 Human)
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  8. FGD5

    Plasmid
    #28109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FGD5:HPC09X-H08:C212499 (FGD5 Human)
    Tags
    His-tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 889-1304.
    Available Since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. KCNK2

    Plasmid
    #73257
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCNK2 (KCNK2 Human)
    Tags
    TEV-His10-FLAG
    Expression
    Insect
    Mutation
    userdefined:Full_Length 1411
    Promoter
    Polyhedrin
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73257-plasmid-map-sequence-id-150197

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  10. CBX2

    Plasmid
    #25158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX2_04 (CBX2 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25158-plasmid-map-sequence-id-472908

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  11. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. GALTA-c001

    Plasmid
    #79645
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GALT (GALT Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79645-plasmid-map-sequence-id-150209

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  13. p-dCas9-DNMT3a-Hygro

    Plasmid
    #104403
    Purpose
    transient expression of dCas9-DNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    DNMT3a (DNMT3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104403-plasmid-map-sequence-id-204468

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  14. pBabe H-Ras V12 C40

    Plasmid
    #18747
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 C40 (HRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    V12 C40
    Available Since
    July 7, 2008
    Availability
    Academic Institutions and Nonprofits only

  15. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  16. pBabe H-Ras V12 S35

    Plasmid
    #18746
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 S35 (HRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    V12 S35
    Available Since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only

  18. pBabe H-Ras V12 G37

    Plasmid
    #18745
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 C40 (HRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    V12 / G37
    Available Since
    July 7, 2008
    Availability
    Academic Institutions and Nonprofits only

  19. pIVT-myc-T58A-S62D

    Plasmid
    #138540
    Purpose
    For making myc-T58A-S62D RNA
    Depositor
    Carmen Williams
    Insert
    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    Use
    In vitro transcription
    Mutation
    Threonine 58 changed to alanine and serine 62 cha…
    Promoter
    T7
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138540-plasmid-map-sequence-id-291746

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  20. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Available Since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

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Showing: 1281 - 1300 of 167150 results