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pLRD18

Plasmid

#208270

Purpose

Production of ent-isokaurene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

ptmT2
osKSL6

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD12

Plasmid

#208264

Purpose

Production of miltiradiene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
smKAL

Expression

Bacterial

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET-SUMO mCER-Prx2

Plasmid

#158226

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

Depositor

Tobias Dick

Publication

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Bacterial

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB4613

Plasmid

#126752

Purpose

Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

Depositor

Michael Jensen

Publication

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

CDC14 (CDC14 Budding Yeast)

Use

Integrative plasmid

Expression

Yeast

Promoter

pTRX2_5xUAS

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Publication

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMXS_IRES_GFP_FLAG-33A1

Plasmid

#257599

Purpose

Expression of FLAG-tagged human SLC33A1 with GFP reporter

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

FLAG-SLC33A1 (SLC33A1 Human)

Expression

Mammalian

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C192A-Myc

Plasmid

#201168

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Publication

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD05

Plasmid

#208259

Purpose

Production of isopimara-8,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
sare_1287

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pICH47742:PtFCP:Cas9YFP

Plasmid

#104894

Purpose

Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

Depositor

Thomas Mock

Publication

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR; ; golden gate assembly

Tags

YFP

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Publication

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPD2X

Plasmid

#86967

Purpose

Expresses PCNA2 protein fused phosphite dehydrogenase and putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Publication

Watanabe et al ChemCatChem 2013 Dec;5(12):3835-3840

Insert

PCNA2 fused to phosphite dehydrogenase and putidaredoxin

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, C73S/C85S in PdX

Promoter

T7 promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB602-TP-mCherry-UnaG

Plasmid

#203483

Purpose

For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused mCherry-UnaG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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