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pFBD-Akt1(K14A)

Plasmid

#86562

Purpose

Expresses human full-length Akt1(K14A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

K14A mutation in the PH domain

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1-RAMP4

Plasmid

#139863

Purpose

Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1-RAMP4 (UFL1 Human)

Use

Lentiviral

Tags

HA

Mutation

recombinant fusion of UFL1 (full length) with RAM…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 ECD

Plasmid

#113954

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-568

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mCit-Snap25b D179K A199R

Plasmid

#53259

Purpose

SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Mutation

D179K and A199R

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only
pJRTCLCrVA009

Plasmid

#22842

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing CHLI fragment

Use

Cotton vigs vector

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTet02

Plasmid

#78284

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro-Arpp19-short

Plasmid

#248798

Purpose

Overexpression of ARPP19 short

Depositor

Maayan Salton

Insert

ARPP19 short isoform (ARPP19 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro-Arpp19-long

Plasmid

#248799

Purpose

Overexpression of ARPP19 long

Depositor

Maayan Salton

Insert

ARPP19 long isoform (ARPP19 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_erbb2_cmlc2-nmKate

Plasmid

#238409

Purpose

Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-Rim1[20-227]-mCherry

Plasmid

#246282

Purpose

Rab10 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rim1[20-227]-mCherry (Rims1 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mVenus-rRim1[20-227]-mVenus(L68V)

Plasmid

#246280

Purpose

Rab10 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

Venus-rRim1[20-227]-mVenus(L68V) (Rims1 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-Rims2[27-179]-mCherry

Plasmid

#246271

Purpose

Rab8 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rims2[27-179]-mCherry (Rims2 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-Rabenosyn5[439-503]-mCherry

Plasmid

#246251

Purpose

Rab4 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rabenosyn5[439-503]-mCherry (Zfyve20 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

Plasmid

#225438

Purpose

Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-FRT-OCS-BeYDVRepRepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

Plasmid

#225437

Purpose

Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-att-OCS-BeYDVRep/RepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

Plasmid

#225430

Purpose

OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-FRT-OCSterm-FRT-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

Plasmid

#225431

Purpose

OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-att-OCSterm-att-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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