We narrowed to 1,322 results for: pET28a

1. pET28-p53 (1-73) (K24N)

   Plasmid

   #62079

   Purpose

   Expression of human p53 (1-73) (K24N) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; K24N

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG87 FAST

   Plasmid

   #130719

   Purpose

   Expresses His-tagged FAST (also called YFAST) in bacteria

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDL52 (clone45)

   Plasmid

   #188578

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDL52 (clone76)

   Plasmid

   #188579

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Met1 diUb (1-152)

   Plasmid

   #110766

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-p53 (1-90) (72P)

   Plasmid

   #62080

   Purpose

   Expression of human p53 (1-90) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD/PP residues 1-90; 72P

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET28-mHP1alpha

   Plasmid

   #181911

   Purpose

   Bacterial protein expression vector for HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mature ISG15 C-term. domain (79-157)

   Plasmid

   #110764

   Purpose

   Bacterial expression of proISG15 C-term. domain

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. proISG15 C-term. domain (79-165)

    Plasmid

    #110763

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His6-SMT3-SopB(C460S)

    Plasmid

    #183677

    Purpose

    Recombinant Salmonella Typhimurium SopB (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    C460S

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28:LanIV-Chondromyces

    Plasmid

    #225078

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Chondromyces)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28:LanII-56

    Plasmid

    #225073

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-56)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28:LanI-101

    Plasmid

    #225065

    Purpose

    Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanI-101)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28:LanII-2E

    Plasmid

    #225072

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2E)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28:LanII-2D

    Plasmid

    #225071

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2D)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His6-EGFP-cPHx1

    Plasmid

    #183674

    Purpose

    PtdIns(3,4)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 169-329

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-His10-BTLAicd (AA190-289)-TwinStrep

    Plasmid

    #180805

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289)-TwinStrep (BTLA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Met1 diUb (1-152, R42W)

    Plasmid

    #110767

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    R42W

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge