We narrowed to 1,332 results for: pET28a

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pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIII-6

Plasmid

#225076

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanIII-6)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-23

Plasmid

#225067

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-23)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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LARP1_323-509

Plasmid

#209800

Purpose

Bacterial expression of human LARP1 La-module

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 La-module (LARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-509 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LARP1_323-410

Plasmid

#209801

Purpose

Bacterial expression of human LARP1 LaM domain

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 LaM (LARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-410 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mePOU-POU

Plasmid

#206392

Purpose

Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSN554

Plasmid

#177361

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

Leucine zipper, StrepII tag, and mScarlet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389H

Plasmid

#137658

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389H

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389W

Plasmid

#137657

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389W

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (K24N)

Plasmid

#62079

Purpose

Expression of human p53 (1-73) (K24N) in e. coli

Depositor

Gary Daughdrill

Article

Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-73; K24N

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pAG87 FAST

Plasmid

#130719

Purpose

Expresses His-tagged FAST (also called YFAST) in bacteria

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

FAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDL52 (clone76)

Plasmid

#188579

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDL52 (clone45)

Plasmid

#188578

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152)

Plasmid

#110766

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28-mHP1alpha

Plasmid

#181911

Purpose

Bacterial protein expression vector for HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. domain (79-157)

Plasmid

#110764

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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proISG15 C-term. domain (79-165)

Plasmid

#110763

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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His6-SMT3-SopB

Plasmid

#183676

Purpose

Recombinant Salmonella Typhimurium SopB

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)

Tags

His6-S. cerevisiae SMT3(2-101)

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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