We narrowed to 6,263 results for: wus

1. p23-mNeongreen-DDX21 DAT

   Plasmid

   #179229

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Mammalian

   Mutation

   G234D, K235A

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. human ETV6 gRNA-4

   Plasmid

   #133404

   Purpose

   human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

   Depositor

   Linzhao Cheng

   Article

   Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

   Insert

   ETV6 (ETV6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas-Cas12m-ΔZF

   Plasmid

   #192276

   Purpose

   Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   Cas12m ΔZF
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   H459A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas-dCas12m-ΔZF

   Plasmid

   #192277

   Purpose

   pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m ΔZF
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D485A, H549A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas_4(I-U)/1_2_VB

   Plasmid

   #186445

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas4 domain swapped with Cas4 domain from G. sulf…

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKate2-DrCav1 in pT3TS-Dest

   Plasmid

   #194293

   Purpose

   In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   caveolin (cav1.S Frog)
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mKate2-DrCavin1a in pT3TS-Dest

   Plasmid

   #194294

   Purpose

   In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   cavin1a (cavin1a Zebrafish)
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E-HsNRF2 (NFE2L2)

   Plasmid

   #194302

   Purpose

   Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   NFE2L2 (NFE2L2 Human)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-DrCavin1a

   Plasmid

   #194291

   Purpose

   Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   cavin1a (cavin1a Zebrafish)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p23-mRuby3-DDX21 HGV

    Plasmid

    #179233

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSIN-CD45

    Plasmid

    #175943

    Purpose

    knockout mouse CD45

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    CD45 gRNA (Ptprc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSIN-Gapdh

    Plasmid

    #175938

    Purpose

    knockout mouse Gapdh

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Gapdh gRNA (Gapdh Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCCL-CellCycle

    Plasmid

    #132429

    Purpose

    Expressing Fucci cell cycle indicator in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    hCdt, hGeminin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pL99

    Plasmid

    #78577

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    inducible PnitA-NitR system

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSAB35

    Plasmid

    #175787

    Purpose

    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

    Depositor

    John Rubinstein

    Article

    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

    Insert

    SidK (1-278)
    

    Use

    Tags

    3xFLAG and 6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-fMCP

    Plasmid

    #238908

    Purpose

    Contains EGFP-fMCP fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

    Insert

    EGFP-fMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET42b-AncBE4max

    Plasmid

    #165157

    Purpose

    Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    AncBE4max
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ABE-RA3.0

    Plasmid

    #208301

    Purpose

    Expresses ABE-RA3.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA3.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. nls-NgAgo-GK

    Plasmid

    #78253

    Purpose

    Mammalian expression of NgAgo (Argonaute from N. gregoryi).

    Depositor

    Chunyu Han

    Article

    Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    NgAgo
    

    Use

    Tags

    a nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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