162,717 results

1. TARDBP_Halo_N_allele

   Plasmid

   #178131

   Purpose

   Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (TARDBP Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71294

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   GNSTM glycosylation motif, HA, Lamp2 signal pepti…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. SARS-CoV-2 S HexaPro

   Plasmid

   #154754

   Purpose

   Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

   Depositor

   Jason McLellan

   Article

   Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

   Insert

   Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCAMBgfp

   Plasmid

   #206130

   Purpose

   This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

   Depositor

   Anne Osbourn

   Article

   Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

   Insert

   hygromycin resistance gene and the Promoter of ToxA+SGFP
   

   Use

   Binary vector for expression of sgfp in filamento…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p405MET25

   Plasmid

   #15961

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MET25 promoter is between SacI and XbaI restricti…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-CBP core

   Plasmid

   #179543

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-eYFP

   Plasmid

   #104055

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBJG1 T7-8xHis-OGT

   Plasmid

   #154271

   Purpose

   Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   bacterial codon optimized

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKGmDUET-NgBR/hCIT

    Plasmid

    #203173

    Purpose

    Expression of human cis-prenyltransferase complex for purification

    Depositor

    William Sessa

    Article

    Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

    Inserts

    NUS1 (NUS1 Human)
    DHDDS (DHDDS Human)
    

    Use

    Tags

    Strep-Tag II-TEV and internally 6HIS tagged

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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