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pGP-AAV-syn-jGCaMP7b-WPRE

Plasmid

#104489

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has service

AAV Retrograde and AAV1

Insert

jGCaMP7b

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pcDNA3.1 Flag-HIF1B (#930)

Plasmid

#99916

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

Insert

HIF1B (ARNT Human)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCold-I-Dsup

Plasmid

#90021

Purpose

To express His-tagged Dsup in bacteria

Depositor

Takekazu Kunieda

Publication

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

His

Expression

Bacterial

Promoter

cspA

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223_PIK3CA_p.H1047R

Plasmid

#82824

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

H1047R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Publication

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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sgOpti

Plasmid

#85681

Purpose

Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

Depositor

Eric Lander, David Sabatini

Publication

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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Lenti-AsCpf1-Blast

Plasmid

#84750

Purpose

Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Hyongbum Kim

Publication

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

AsCpf1

Use

CRISPR and Lentiviral

Tags

NLS-3xHA (C terminal on insert)

Expression

Mammalian

Promoter

EFS-NS

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

Plasmid

#85039

Purpose

For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

Depositor

Takuji Iwasato

Publication

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

RFP-P2A-tTA

Use

AAV

Expression

Mammalian

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLJM5-WT PHGDH

Plasmid

#83901

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBIG1a

Plasmid

#80611

Purpose

pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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