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Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterhH1Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_mU6
Plasmid#102845PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterActin5CAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB3 AurA
Plasmid#68270Purposebacterial expression of human AurADepositorInsertAurA (AURKA Human)
UseTagsMxe intein/chitin binding domainExpressionBacterialMutationSee depositor comment belowPromoterT7Available sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXN3
Plasmid#153139PurposeExpression of Flag tagged human FOXN3 in mammalian cellsDepositorInserthuman FOXN3 (FOXN3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_hH1
Plasmid#102847PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterU6Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMG
Plasmid#138264PurposeYeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.DepositorInsertseGFP flanked by iCas9 sites
mCherry
UseCRISPRTagsExpressionYeastMutationPromoterTef1Available sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR01-FnCpf1
Plasmid#176529PurposeNuclease Cpf1 adapted for Aspergillus speciesDepositorInsertFnCpf1
UseCRISPR; Fungal expressionTagsExpressionMutationPromoterTEF1Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-5'/3'ss mut
Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation5' and 3' splice sites mutationsPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_h7SK
Plasmid#102846PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_h7SK
UsePcr template for stagr insertsTagsExpressionMutationPromoterH7SKAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BACK))
Plasmid#169722PurposeMammalian Expression of mouse SPOP Y353EDepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationY353EPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR4_SNORD27 probe
Plasmid#73069Purposeantisense probe for detection of human SNORD27DepositorInsertSNORD27 (SNORD2 Human)
UseIn vitro transcriptionTagsExpressionMutationPromotert7Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-3'ss mut
Plasmid#67644PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation3' splice site muattionPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYG026
Plasmid#65328Purposereporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.DepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
E547* FL USP7
Plasmid#229001PurposeE547-mutant of human USP7DepositorInsertUSP7 1-546 (USP7 Human)
UseTags6xHis cleavable with thrombinExpressionBacterialMutationE547 mutated to stop codonPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only