We narrowed to 17,638 results for: URE

Showing: 1301 - 1320 of 17638 results

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pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
mVenus-synaptojanin1(D730A,498-901)-CAAX

Plasmid

#241304

Purpose

Mammalian expression of truncated form of 5-phosphatase-dead synaptojanin-1 with CAAX motif fused to mVenus

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Synaptojanin-1 (SYNJ1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

498-901 aa, D730A

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Lyn-FRB_star-P2A-mVenus-FKBP-5ptase

Plasmid

#241305

Purpose

Mammalian expression of Lyn-FRB_star and mVenus-FKBP-5ptase of INPP5E

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

INPP5E (INPP5E Human)

Tags

mVenus, FKBP

Expression

Mammalian

Mutation

214-644 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mVenus-synaptojanin1(498-901)-CAAX

Plasmid

#241303

Purpose

Mammalian expression of truncated form of synaptojanin-1 with CAAX motif fused to mVenus

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Synaptojanin-1 (SYNJ1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

498-901 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pANAP-P2A-eRF(E55D)

Plasmid

#241290

Purpose

Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

the amber suppressor tRNACUA-Leu

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW281_dSpRY

Plasmid

#244822

Purpose

Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CAG

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS

Plasmid

#244835

Purpose

Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

Plasmid

#177365

Purpose

AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Inserts

tdTomato
Chimeric guide for SaCas9

Use

AAV

Tags

P2A and a tetracycline-controlled transactivator …

Expression

Mammalian

Promoter

CMV and U6 promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Palpha2

Plasmid

#204441

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

W771A K772A K773A R774A W775A L776A Y777A W778A K…

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a Sa-iPGM-bio

Plasmid

#179523

Purpose

Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

S. aureus iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 S3070F

Plasmid

#139325

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 S3070F using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 S1363L

Plasmid

#139329

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 S1363L using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 E1754K

Plasmid

#139330

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E1754K using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA FANCD2 Q223Stop

Plasmid

#139332

Purpose

Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert FANCD2 Q223Stop using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GABARα6N-HA

Plasmid

#128111

Purpose

Express HA tagged GABARα6 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra6 (Gabra6 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-33/myc-His

Plasmid

#127492

Purpose

Expresses myc-tagged _MTS NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-33 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-33

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-78/myc-His

Plasmid

#127493

Purpose

Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-78 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-78

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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