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pTCAV-FLEx(loxP)-FlpO

Plasmid

#67829

Purpose

Cre-dependent expression of FlpO from CAV2 virus

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

FlpO

Expression

Mammalian

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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peZinCh-2

Plasmid

#70008

Purpose

expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

eZinCh-2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox3

Plasmid

#58341

Purpose

expresses human Nox3 in mammalian cells

Depositor

Botond Banfi

Article

Kiss et al Curr Biol. 2006 Jan 24;16(2):208-13.

Insert

NADPH oxidase 3 (NOX3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Squash

Plasmid

#177913

Purpose

Expresses Squash aptamer in mammalian cells using the U6+27 promoter.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Squash aptamer

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Ii-Str_puromycin

Plasmid

#65309

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBac-DsRed-ORCO_9kbProm-QF2

Plasmid

#104877

Purpose

Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

AgORCO-QF2

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET_GFP-SZ2

Plasmid

#124931

Purpose

Expression of GFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

GFP-SYNZIP2

Tags

GFP and His

Expression

Bacterial

Mutation

SZ2 domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g2 Cas9-T2A-mCherry

Plasmid

#164253

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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MRAS

Plasmid

#55661

Purpose

expression of mouse MRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

MRAS (Mras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCMVMagFRET-1-NLS

Plasmid

#50748

Purpose

Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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