-
-
pAAV-U6-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120392PurposepAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoterDepositorTypeEmpty backboneUseAAVTagsmCherry.3XFLAGExpressionMutationPromoterU6Available sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA ADAR1 p150
Plasmid#237855PurposeLentiviral expression of ADAR1 p150DepositorInsertADAR1 (ADAR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA ADAR1 p110
Plasmid#237854PurposeLentiviral vector expressing ADAR1 p110DepositorInsertADAR1 (ADAR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-CIBN-GFP
Plasmid#79572Purposeexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFPDepositorInsertLyn11
UseTagsCIBN (CRY2-binding domain) and EGFPExpressionMammalianMutationG8R (please see depositor comments below)PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLARRY-EGFP
Plasmid#140025PurposeLentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNADepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AbKRS-chIPYE
Plasmid#140020PurposePlasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
UseTagsFLAGExpressionMammalianMutation1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…PromoterEF1Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only
