165,385 results

1. YFVdelCME-mVenus

   Plasmid

   #179953

   Purpose

   pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking C, prM, E proteins but with mVenus
   

   Use

   Transneuronal tracing

   Promoter

   SP6

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG5 Large T

   Plasmid

   #9053

   Depositor

   William Hahn

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1-3xFLAG-HSP27

   Plasmid

   #129229

   Purpose

   Encodes FLAG tagged human HSP27 for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   HSP27 (HSPB1 Human)
   

   Tags

   FLAG and GST

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAY5 (mAID-EGFP-NLS piggyBac)

   Plasmid

   #140532

   Purpose

   mAID-EGFP-NLS piggyBac

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRADZ1

   Plasmid

   #47698

   Purpose

   General cloning vector for use in E. coli or D. radiodurans with lacZ

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   repU

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. enIscB-T5E

   Plasmid

   #205411

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
   

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FU-H2B-GFP-IRES-Puro

   Plasmid

   #69550

   Purpose

   Lentiviral, selectable fluorescent GFP tagging of nuclei.

   Depositor

   Charles Gersbach

   Insert

   Histone 2B - green fluorescent protein fusion
   

   Use

   Lentiviral

   Tags

   GFP fusion

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro U6 sgRNA BfuAI stuffer

    Plasmid

    #50920

    Purpose

    U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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