170,823 results

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pAAV-hSyn-rtTA3G-TetOn3G

Plasmid

#250672

Purpose

Drive Tet-ON activator expression from human synapsin promoter for doxycycline-inducible transgene activation

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

rtTA3G-TetOn3G

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-U6:gRNA

Plasmid

#63156

Purpose

Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC38A7_STOP

Plasmid

#161260

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A7 (SLC38A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-hMeCP2-GFP

Plasmid

#48078

Purpose

Mammalian expression of GFP tagged MeCP2-WT

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsEDC4_S

Plasmid

#147455

Purpose

Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsEDC4 (EDC4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

Plasmid

#196084

Purpose

(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Inserts

dCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold

Use

CRISPR

Expression

Mammalian

Mutation

Catalytically dead mutant of the Cas9 endonucleas…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn1-FLEX-hChR2-tdtomato

Plasmid

#41015

Depositor

Karel Svoboda

Insert

Channelrhodopsin-2

Use

Adenoviral

Tags

tdtomato

Expression

Mammalian

Mutation

codon-optimized (human)

Promoter

synapsin

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only