172,032 results

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tCRMod-GGSGGS9

Plasmid

#111762

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS9-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCK306

Plasmid

#110544

Purpose

rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

PrhaBAD
yfp
rhaS

Expression

Bacterial

Promoter

N/A, PrhaBAD, and kanR promoter (upstream of kanR…

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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∆DUSP6

Plasmid

#27977

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

C293S: mutation in the phosphatase domain

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

Plasmid

#196936

Purpose

TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

Depositor

Elaine Fuchs

Article

Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

Insert

mCherry

Use

Lentiviral and RNAi

Tags

P2A-CreERT2

Expression

Mammalian

Promoter

SBE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +0

Plasmid

#66939

Purpose

CRISPaint frame selector +0

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +0

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

Plasmid

#196829

Purpose

Express dpdpCas13b with AAAA mutation and Clover

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Tags

3XFLAG and NES

Expression

Mammalian

Mutation

K367A, D369A and K370A

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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