We narrowed to 22,307 results for: CRISPR

1. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh and U6

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

   Plasmid

   #159903

   Purpose

   Mutagenesis of Oprk1

   Depositor

   Larry Zweifel

   Insert

   Oprk1 (Oprk1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEG302-SunTagng-22aa

   Plasmid

   #115345

   Purpose

   Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome

   Depositor

   Steven Jacobsen

   Insert

   NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgNts

   Plasmid

   #159909

   Purpose

   Mutagenesis of Nts

   Depositor

   Larry Zweifel

   Insert

   Nts (Nts Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJH2972

   Plasmid

   #100956

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH2971

   Plasmid

   #100955

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH2970

   Plasmid

   #100954

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

   Plasmid

   #159920

   Purpose

   Mutagenesis of Vamp2

   Depositor

   Larry Zweifel

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TMPRSS2 g1 + Cas9

   Plasmid

   #153015

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid

    #104419

    Purpose

    transient expression of dCas9-dp300 fusion protein

    Depositor

    Stephan Beck

    Insert

    dp300
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    S1396R-S1397R

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFETCh_ATF4

    Plasmid

    #72350

    Purpose

    Donor vector for 3' FLAG tag of human ATF4

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    ATF4 Homology Arms (ATF4 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TMPRSS2 g4 + Cas9

    Plasmid

    #153018

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZBTB12.1.0-gDNA

    Plasmid

    #113769

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB12

    Depositor

    Kevin White

    Insert

    ZBTB12 (ZBTB12 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZBTB17.1.0-gDNA

    Plasmid

    #112483

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB17

    Depositor

    Kevin White

    Insert

    ZBTB17 (ZBTB17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSPDEF.1.0-gDNA

    Plasmid

    #113796

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEF

    Depositor

    Kevin White

    Insert

    SPDEF (SPDEF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMLX.1.0-gDNA

    Plasmid

    #112466

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MLX

    Depositor

    Kevin White

    Insert

    MLX (MLX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTFCP2.1.0-gDNA

    Plasmid

    #112404

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFCP2

    Depositor

    Kevin White

    Insert

    TFCP2 (TFCP2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQdCas12a.sggfp(B)

    Plasmid

    #236040

    Purpose

    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQdCas12a.sggfp(C)

    Plasmid

    #236042

    Purpose

    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psBIG1a

    Plasmid

    #229957

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. psBIG1b

    Plasmid

    #229958

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. psBIG2abc

    Plasmid

    #229963

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. psBIG2ab

    Plasmid

    #229962

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. psBIG2abcd

    Plasmid

    #229964

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. psBIG2abcde

    Plasmid

    #229965

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. psBIG1d

    Plasmid

    #229960

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. psBIG1e

    Plasmid

    #229961

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. psBIG1c

    Plasmid

    #229959

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

    Plasmid

    #199496

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330.orup

    Plasmid

    #110404

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge