-
-
pDEST15.1-hRPN13 (285-407)
Plasmid#61934PurposeExpression of hRPN13 (285-407) in E.coliDepositorInserthRPN13 (285-407) (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationConstruct starts at residue 285PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) pCMV
Plasmid#8539DepositorInsertHOXB7 (HOXB7 Human)
UseTagsFLAGExpressionMammalianMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Kif5cmut
Plasmid#71854Purposemammalian expression of mutant GFP-Kif5cDepositorInsertKif5c (Kif5c Rat)
UseTagsEGFPExpressionMammalianMutationT93N mutation and also a novel SpeI site (1652). …PromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mPA-GFP-PA-Rac1
Plasmid#22032DepositorInsertmPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3
Plasmid#49822Purposefor transcribing SRA nucl 419-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(767-1356)
Plasmid#49826Purposefor transcribing SRA nucl 767-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT259_(pRosa26-G-tTA2)
Plasmid#36878DepositorInsertsGFP
tTA2
beta-globin intron
Neo
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 ATG wt
Plasmid#52034Purposeencodes wt HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationstarting ATG deletedPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T3-ZO-2-1st-PDZ-delta-NLS
Plasmid#27425DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationDeletion of NLS-Nuclear Localization Signal: Nucl…PromoterAvailable sinceMay 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(1000-1356)
Plasmid#49827Purposefor transcribing SRA nucl 1000-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
2.3 hA33 luciferase
Plasmid#68148Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -2270 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain EE mutation
Plasmid#58833PurposeExpresses GFP-Tagged and mutated CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationchanged Arginine 43 to GlutamatePromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPSt_35Spr-GFP-t35S
Plasmid#203592PurposeFully assembled Plant STARR-seq plasmid with the 35S terminator.DepositorInsertsCaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1243 - pL0_pT3O (pro + 5U)
Plasmid#203891PurposeT3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertT3O promoter and 5'UTR
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1198 - pL0_pD4H (pro + 5U)
Plasmid#203894PurposeD4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertD4H promoter and 5'UTR
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2701-AGER-tdTomato
Plasmid#216470Purposedonor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start siteDepositorInserttdTomato
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLINE1mORF1-ΔORF2-scFvFc
Plasmid#206023PurposeThe base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Gja1prom
Plasmid#188114PurposeExpresses luciferase under control of Gja1 promoter in transfected cellsDepositorInsertGja1 promoter (Gja1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterGja1 endogenous promoter up to gene start codonAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
1.2 hA33 luciferase
Plasmid#68149Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -1149 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.6 hA33 luciferase
Plasmid#68150Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -595 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.44 hA33 luciferase
Plasmid#68151Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -443 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.42 hA33 luciferase
Plasmid#68152Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -423 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.41 hA33 luciferase
Plasmid#68154Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -405 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.36 hA33 luciferase
Plasmid#68155Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -363 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.38 hA33 luciferase
Plasmid#68156Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -384 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.22 hA33 luciferase
Plasmid#68157Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -222 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(419-999)
Plasmid#49824Purposefor transcribing SRA nucl 419-999 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(767-999)
Plasmid#49825Purposefor transcribing SRA nucl 767-999 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(419-771)
Plasmid#49823Purposefor transcribing SRA nucl 419-771 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
UseTagsExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-fLuc
Plasmid#40589DepositorInserthGFAP promoter (GFAP Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationContains human GFAP promoter fragment (-2163 to +…PromoterGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)
Plasmid#221746PurposeStable expression of GFP-CERT(PHD)in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_KLF4
Plasmid#26815DepositorInsert5'UTR-KLF4-3'UTR (KLF4 Human)
UseTagsExpressionMammalianMutationStarts from second ATGPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag Depdc5 pRK5
Plasmid#46340DepositorInsertDepdc5 (DEPDC5 Human)
UseTagsFlagExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorInsertDAP5 (EIF4G2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -