We narrowed to 7,333 results for: ef1a
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Plasmid#198392PurposeRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site double mutant (K107/113R)PromoterEF1AAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSPromoterEF1a and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-tagBFP-NLS-3xFlag
Plasmid#236385PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag
Plasmid#236364PurposeOverexpression of dLbCas12a in human cellsDepositorInsertdLbCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3xFlag
Plasmid#236367PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbCas12aRVRR-EGFP-NLS-3xFlag
Plasmid#236365PurposeOverexpression of dLbCas12aRVRR in human cellsDepositorInsertdLbCas12aRVRR
UseCRISPR and LentiviralMutationG532R, K538V, Y542R, K595RAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL163
Plasmid#243686PurposeStably expresses ALDH18A1 CDS with its endogenous 5'UTR and 3'UTRDepositorAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL179
Plasmid#243688PurposeStably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1DepositorAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-Parkin
Plasmid#237397PurposeLentivirus plasmid for the stable expression of mCherry-Parkin in mammalian cellsDepositorAvailable SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag
Plasmid#236376PurposeOverexpression of HyperdFnCas12a in human cellsDepositorInsertHyperdFnCas12a
UseCRISPR and LentiviralMutationE184R, T279R, D340R, D409RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag
Plasmid#236375PurposeOverexpression of dFnCas12a in human cellsDepositorInsertdFnCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag
Plasmid#236363PurposeOverexpression of endAsCas12a in human cellsDepositorInsertendAsCas12a
UseCRISPR and LentiviralMutationE174R, S542R, K548RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag
Plasmid#236362PurposeOverexpression of HyperdAsCas12a in human cellsDepositorInsertHyperdAsCas12a
UseCRISPR and LentiviralMutationE174R, D256R, E321R, E371RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag
Plasmid#236361PurposeOverexpression of dAsCas12a in human cellsDepositorInsertdAsCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_-DIO-Varnam1-WPRE
Plasmid#240054PurposeCre dependent Red fluorescent voltage indicator for mammalian expressionDepositorInsertVARNAM1
UseAAVPromoterEf1aAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFE-mSG-Puro
Plasmid#226522PurposeCas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-PuroDepositorInserthU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro
UseCRISPR and LentiviralTagsmStayGoldExpressionMammalianPromoterU6,EF1aAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-TET1-dCas9
Plasmid#235599PurposeExpresses Tet1CD-dCas9DepositorInsertTET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
UseCRISPR and LentiviralTags3xNLS and TET1 CDExpressionMammalianPromoterEF1aAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-PTPN11
Plasmid#215523PurposeExpression vector with Shp2DepositorAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF.DEST51-PTPN11-Clover
Plasmid#215525PurposeExpression vector with clover-tagged Shp2DepositorAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)
Plasmid#223146PurposeExpression of truncated NIPP1 with dCjCas9 and empty gRNA scaffoldDepositorInsertNIPP1 (PPP1R8 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 143-224PromoterEF1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)
Plasmid#223156PurposeExpression of truncated NIPP1 with dSaCas9 and empty gRNA scaffoldDepositorInsertNIPP1 (PPP1R8 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 143-224PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A
Plasmid#204622PurposeSingle-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.DepositorInsertmRFP1
ExpressionMammalianPromoterEF1aAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v2
Plasmid#178536PurposeSubcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backboneDepositorAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v1
Plasmid#178535PurposeSubcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backboneDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmx4-V5 C209A
Plasmid#175140PurposeLentiviral expression of V5-tagged mouse Tmx4DepositorAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmx4-V5 C211A
Plasmid#175139PurposeLentiviral expression of V5-tagged mouse Tmx4DepositorAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Hsd11b1-V5
Plasmid#175147PurposeLentiviral expression of mouse Hsd11b1-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCh-633
Plasmid#92253PurposePlasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
TagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterEF1AAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-TSAd-Y89XF
Plasmid#84559Purposemammalian expression of TSAd-Y89XFDepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.mNeon
Plasmid#69146PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGPromoterEFS and hU6Available SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ENL)
Plasmid#69212PurposeLentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])DepositorInsertsUseCRISPR and LentiviralTagsFLAGAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.PAC
Plasmid#57828PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHIV-IL13-GAPGlySer-PDGFR
Plasmid#240247PurposeLentiviral transfer plasmid encoding IL13 with GAP-Gly-Ser linker and PDGFR TMDepositorAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)
Plasmid#197892PurposeCan be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInserthM4D/2A/GFP
UseAAVMutationN/APromoterEF1aAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only