We narrowed to 1,878 results for: Ipp

1. pLV_dCas9-KRAB_sgSTK3i_#1 (PuroR)

   Plasmid

   #209771

   Purpose

   CRISPRi for STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK3i (STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

   Plasmid

   #204620

   Purpose

   Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-Lamp1 FLAG

   Plasmid

   #199750

   Purpose

   Lentiviral construct to stably express Lamp1

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Lamp1 (Lamp1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-myc Metap2

   Plasmid

   #199685

   Purpose

   Transient expression of Metap2

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN687

   Plasmid

   #177363

   Purpose

   Expresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tag

   Depositor

   Shinsuke Niwa

   Article

   Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

   Insert

   KIF1A (KIF1A Human)
   

   Use

   Tags

   His tag and Leucine zipper

   Expression

   Bacterial

   Mutation

   changed Proline 305 to Leucine

   Promoter

   T7

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET-osr1-KI-donor

   Plasmid

   #184875

   Purpose

   Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

   Depositor

   Christoph Winkler

   Article

   Hui et al Development. 2022 May 20. pii: 275483. d

   Inserts

   Odd-Skipped Related Transcription Factor 1 5' homology arm
   Odd-Skipped Related Transcription Factor 1 3' homology arm
   p2a-CreERT2
   Myosin light chain 2 promoter
   EGFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmirGlo-RBM8A-3'UTR 240-546

   Plasmid

   #158108

   Purpose

   reporter 3'UTR hRBM8A (short)

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   3'UTR of hRBM8A (RBM8A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

   Plasmid

   #174133

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   Only contains the sequences coding for amino acid…

   Promoter

   hSyn

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

   Plasmid

   #174135

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   Only contains the sequences coding for amino acid…

   Promoter

   hSyn

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCh-646

    Plasmid

    #92289

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 CED 7W7A (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215 with the following point …

    Promoter

    Ac5

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdEos-EB3-7

    Plasmid

    #57610

    Purpose

    Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Use

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

    Plasmid

    #170226

    Purpose

    Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    IKBKB (IKBKB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    S177E, S181E

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid

    #170236

    Purpose

    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S176E, S180E

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

    Plasmid

    #170237

    Purpose

    Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHLS-EF1a-FRB-SpCas9-A

    Plasmid

    #138477

    Purpose

    Expresses FRB fused SpCas9 for NanoMEDIC packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    SpCas9, human codon optimized
    

    Use

    CRISPR

    Tags

    3x HA tag and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-ARAP3

    Plasmid

    #39484

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3 (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only