-
Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
UseTagsExpressionBacterial and YeastMutationQ773A R775A R776A Q778APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT1
Plasmid#67116PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT1 (STAT1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1L
Plasmid#67096PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2A1L (GTF2A1L Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TP53
Plasmid#67078PurposeStandard for cell-free expression benchmarking.DepositorInsertTP53 (TP53 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IRF9
Plasmid#67079PurposeStandard for cell-free expression benchmarking.DepositorInsertIRF9 (IRF9 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 E-Cad
Plasmid#67046PurposeStandard for cell-free expression benchmarking.DepositorInsertE-Cad (CDH1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCEB2
Plasmid#67049PurposeStandard for cell-free expression benchmarking.DepositorInsertTCEB2 (ELOB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationM6R and Q115KPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BTF3L4
Plasmid#67051PurposeStandard for cell-free expression benchmarking.DepositorInsertBTF3L4 (BTF3L4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hHSF1 WT
Plasmid#71724Purposeplasmid expressing myc-his tag hHSF1 WTDepositorInsertHSF1 (HSF1 Human)
UseTagsMYC 6xHISExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJEP300-pAAV-CMV-MCS2-pA
Plasmid#112138PurposeCMV driven Multi Cloning Site-2DepositorInsertN/A
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BRD4
Plasmid#183939PurposeTranscriptional co-activator BRD4 labeled by mCherryDepositorInsertmCherry-BRD4 (Brd4 Synthetic, Mouse)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralTagsExpressionMutationPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[30Q]
Plasmid#33236DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsFlagExpressionMammalianMutationinsert contains a stretch of 29 amino acids (27 Q…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAS9PBKS
Plasmid#68371PurposeCbAkt promoter driven Cas9-2A-GFP (from px458)DepositorInsertCas9
UseTags2A-EGFPExpressionMammalianMutationPromoterCbAktAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB9v1
Plasmid#210028PurposeContains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puroDepositorInsertsCas9
rtta-T2A-puro
UseCRISPRTagsSV40 NLS and nucleoplasmin NLSExpressionMammalianMutationPromoterEF-1α and TRE3GAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRARE-MBP-DEST
Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
UseTagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6GRNA
Plasmid#68370PurposegRNA scaffold with hU6 promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTHmut
Plasmid#178950PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cellsDepositorInsertAPOBEC1-YTHmut-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[85Q]
Plasmid#33237DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsFlagExpressionMammalianMutationinsert contains a stretch of 85 Glutamines and no…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only