We narrowed to 1,471 results for: cag promoter

1. pU6_HEK3+1T>A_(PP7-C4-Q1)

   Plasmid

   #232437

   Purpose

   pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4440_BioBrick-hsf-1-sgRNA-B

   Plasmid

   #177787

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

   Plasmid

   #218860

   Purpose

   10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hGCG-ICAM(1235)-nrxn1b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

   Plasmid

   #186699

   Purpose

   Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.175

   Plasmid

   #184996

   Purpose

   Express -Eco1 HEK3 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK3 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCL.177

   Plasmid

   #184998

   Purpose

   Express -Eco1 EMX1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   EMX1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL.180

    Plasmid

    #185001

    Purpose

    Express -Eco1 AAVS1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    AAVS1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSuper-PACSIN1-shRNA

    Plasmid

    #72576

    Purpose

    Expresses shRNA against PACSIN1

    Depositor

    Victor Anggono, Richard Huganir

    Article

    Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

    Insert

    PACSIN1 (Pacsin1 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 promoter

    Available since

    May 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA[bTub+DsxF].1046A

    Plasmid

    #112694

    Purpose

    express two gRNA targeting bTub & DsxF under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP

    Plasmid

    #210509

    Purpose

    expresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Inserts

    hSyn promoter (RIC8B )
    PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

    Plasmid

    #184464

    Purpose

    Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPN062

    Plasmid

    #91595

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SunTagCACTA1g2-22aa-TET1cd

    Plasmid

    #106437

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN098

    Plasmid

    #91625

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN099

    Plasmid

    #91626

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge