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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKvenus-Scribble

Plasmid

#58738

Purpose

Expresses Venus-tagged human Scribble protein

Depositor

Ian Macara

Article

Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

Insert

Scribble (SCRIB Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pPY22-mNeonGreen-Hyg

Plasmid

#137082

Purpose

Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

Depositor

Gohta Goshima

Article

Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

Insert

mNeonGreen-Flag

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Use

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP

Plasmid

#179635

Purpose

HCVP-inducible tri-transmembrane SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP

Plasmid

#179638

Purpose

HCVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-cMYC promoter

Plasmid

#114724

Purpose

reporter luciferase construct

Depositor

Martine Roussel

Article

Roussel et al Oncogene. 1994 Feb;9(2):405-15.

Insert

C-Myc promoter region

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP8045

Plasmid

#220900

Purpose

Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

tnsABCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pNR64

Plasmid

#79540

Purpose

Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI

Use

Tags

His tag and LAA degradation tag

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk4

Plasmid

#1874

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pKT-iRFP-KAN

Plasmid

#64687

Purpose

pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

iRFP

Use

Tags

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Promoter

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsz

Plasmid

#109355

Purpose

GsX chimera with Gαz C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsz chimera

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCOLA-Spec-EV

Plasmid

#212095

Purpose

(Empty Backbone) pCOLA-Spec Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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PKC delta DN

Plasmid

#16389

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta isoform 2 (Prkcd Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K376R

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet

Plasmid

#125134

Purpose

Natively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFS461

Plasmid

#89064

Purpose

leu1 integration plasmid with adh1pr:Z3EV

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV artificial transcription factor

Use

Leu1 integrating vector

Tags

Expression

Mutation

Promoter

adh1

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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PKC beta I DN

Plasmid

#16381

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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